Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Biomolecules. 2021 Oct 26;11(11):1583. doi: 10.3390/biom11111583.
Vertical vibration (VV) is a type of whole body vibration, which induces muscle contraction through vibration to improve muscle strength and bone density. However, the mechanism of VV on muscle cell myotube formation is still unclear. In the current study, we aim to clarify the mechanism involved in VV's stimulation of myotube formation. In order to identify the molecules regulated by VV, we performed proteomics analysis including 2D electrophoresis combined with MALDI-TOF/TOF Mass. Stathmin was identified as a high potential molecule responding to VV stimulation, and we found that under VV stimulation, the expression of stathmin gene and protein increased in a time-dependent manner. In addition, we also confirmed that the increase of stathmin stimulated by VV is mediated through the PI3K/Akt pathway. Furthermore, stathmin siRNA significantly down-regulated the expression of myogenic regulatory factor (MRF) MyoD, decorin, and type I collagen (Col-I), and down-regulated the cellular process regulators such as FGF7, TGFBr1 and PAK3. Taken together, our results confirm that under the stimulation of VV, PI3K/Akt and stathmin would be activated, as well as the up-regulation of MRFs, such as FGF7, TGFBr1 and PAK3 to initiate myogenesis. It also showed that the response of MRF to VV stimulation was significantly related to stathmin expression, which also confirmed the importance of stathmin in the entire myotube formation process. This study may provide evidence of stathmin as a biological indicator of VV to increase muscle strength.
垂直振动（VV）是一种全身振动，通过振动诱导肌肉收缩，从而提高肌肉力量和骨密度。然而，VV 对肌细胞肌管形成的作用机制尚不清楚。在本研究中，我们旨在阐明 VV 刺激肌管形成的机制。为了鉴定受 VV 调节的分子，我们进行了蛋白质组学分析，包括 2D 电泳结合 MALDI-TOF/TOF Mass。Stathmin 被鉴定为对 VV 刺激有高反应潜力的分子，我们发现 VV 刺激下，stathmin 基因和蛋白的表达呈时间依赖性增加。此外，我们还证实，VV 刺激引起的 stathmin 增加是通过 PI3K/Akt 途径介导的。此外，stathmin siRNA 显著下调肌生成调节因子（MRF）MyoD、decorin 和 I 型胶原（Col-I）的表达，并下调细胞过程调节剂如 FGF7、TGFBr1 和 PAK3 的表达。总之，我们的结果证实，在 VV 的刺激下，PI3K/Akt 和 stathmin 会被激活，以及 MRFs（如 FGF7、TGFBr1 和 PAK3）的上调，从而启动肌生成。这也表明 MRF 对 VV 刺激的反应与 stathmin 的表达明显相关，这也证实了 stathmin 在整个肌管形成过程中的重要性。本研究可为 stathmin 作为 VV 增加肌肉力量的生物学指标提供依据。
