Xu Jun, Xian Qianqian, Zhang Ningyuan, Wang Ke, Zhou Xin, Li Yansong, Dong Jingping, Chen Xuehao
School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
State Key Laboratory of Vegetable Germplasm Innovation, Tianjin 300192, China.
Biomolecules. 2021 Nov 2;11(11):1620. doi: 10.3390/biom11111620.
wilt () of cucumber ( L.) caused by f. sp. (Foc) is a destructive soil-borne disease that severely decreases cucumber yield and quality worldwide. MicroRNAs (miRNAs) are small non-coding RNAs (sRNAs) that are important for regulating host immunity because they affect target gene expression. However, the specific miRNAs and the miRNA/target gene crosstalk involved in cucumber resistance to remain unknown. In this study, we compared sRNA-seq and RNA-seq data for cucumber cultivar 'Rijiecheng', which is resistant to . The integrated analysis identified -responsive miRNAs and their target genes. On the basis of verified expression levels, we detected two highly expressed miRNAs with down-regulated expression in response to Foc. Moreover, an analysis of 21 target genes in cucumber inoculated with Foc indicated that (), which is targeted by , and (), (), and (), which are targeted by , are expressed at high levels, but their expression is further up-regulated after Foc inoculation. These results imply that -, -, - and - regulate cucumber defenses against , and provide the gene resources that may be useful for breeding programs focused on developing new cucumber varieties with enhanced resistance to .
由尖孢镰刀菌古巴专化型（Foc）引起的黄瓜（Cucumis sativus L.）枯萎病是一种具有毁灭性的土传病害，在全球范围内严重降低黄瓜产量和品质。微小RNA（miRNA）是一类小的非编码RNA（sRNA），因其影响靶基因表达而对调节宿主免疫至关重要。然而，参与黄瓜对Foc抗性的特定miRNA以及miRNA/靶基因的相互作用仍不清楚。在本研究中，我们比较了抗Foc的黄瓜品种‘日结成’的sRNA测序和RNA测序数据。综合分析确定了对Foc响应的miRNA及其靶基因。基于验证的表达水平，我们检测到两个在响应Foc时表达下调的高表达miRNA。此外，对接种Foc的黄瓜中21个靶基因的分析表明，被miR166靶向的CsaV3_4G014780（WOX1）以及被miR156靶向的CsaV3_2G023360（SPL9）、CsaV3_6G014380（SPL10）和CsaV3_6G014400（SPL11）高水平表达，但在接种Foc后其表达进一步上调。这些结果表明，miR166、miR156、SPL9和WOX1调节黄瓜对Foc的防御，并提供了可能有助于专注于培育具有增强Foc抗性的新黄瓜品种的育种计划的基因资源。
