Neiburga Katrīna D, Vilne Baiba, Bauer Sabine, Bongiovanni Dario, Ziegler Tilman, Lachmann Mark, Wengert Simon, Hawe Johann S, Güldener Ulrich, Westerlund Annie M, Li Ling, Pang Shichao, Yang Chuhua, Saar Kathrin, Huebner Norbert, Maegdefessel Lars, Lange Rüdiger, Krane Markus, Schunkert Heribert, von Scheidt Moritz
Bioinformatics Lab, Riga Stradiņš University, LV-1007 Riga, Latvia.
SIA Net-OMICS, LV-1011 Riga, Latvia.
Biomolecules. 2021 Nov 12;11(11):1683. doi: 10.3390/biom11111683.
Cardiovascular disease (CVD) is the leading cause of morbidity and mortality worldwide. Non-coding RNAs have already been linked to CVD development and progression. While microRNAs (miRs) have been well studied in blood samples, there is little data on tissue-specific miRs in cardiovascular relevant tissues and their relation to cardiovascular risk factors. Tissue-specific miRs derived from (IMA) from 192 coronary artery disease (CAD) patients undergoing coronary artery bypass grafting (CABG) were analyzed. The aims of the study were 1) to establish a reference atlas which can be utilized for identification of novel diagnostic biomarkers and potential therapeutic targets, and 2) to relate these miRs to cardiovascular risk factors. Overall, 393 individual miRs showed sufficient expression levels and passed quality control for further analysis. We identified 17 miRs-miR-10b-3p, miR-10-5p, miR-17-3p, miR-21-5p, miR-151a-5p, miR-181a-5p, miR-185-5p, miR-194-5p, miR-199a-3p, miR-199b-3p, miR-212-3p, miR-363-3p, miR-548d-5p, miR-744-5p, miR-3117-3p, miR-5683 and miR-5701-significantly correlated with cardiovascular risk factors (correlation coefficient >0.2 in both directions, -value ( < 0.006, false discovery rate (FDR) <0.05). Of particular interest, miR-5701 was positively correlated with hypertension, hypercholesterolemia, and diabetes. In addition, we found that miR-629-5p and miR-98-5p were significantly correlated with acute myocardial infarction. We provide a first atlas of miR profiles in IMA samples from CAD patients. In perspective, these miRs might play an important role in improved risk assessment, mechanistic disease understanding and local therapy of CAD.
心血管疾病（CVD）是全球发病和死亡的主要原因。非编码RNA已被证明与CVD的发生和发展有关。虽然微小RNA（miRs）在血液样本中已得到充分研究，但关于心血管相关组织中组织特异性miRs及其与心血管危险因素的关系的数据却很少。对192例行冠状动脉旁路移植术（CABG）的冠心病（CAD）患者的内乳动脉（IMA）中衍生的组织特异性miRs进行了分析。该研究的目的是：1）建立一个可用于鉴定新型诊断生物标志物和潜在治疗靶点的参考图谱；2）将这些miRs与心血管危险因素联系起来。总体而言，393个个体miRs表现出足够的表达水平并通过质量控制以进行进一步分析。我们鉴定出17个miRs——miR-10b-3p、miR-10-5p、miR-17-3p、miR-21-5p、miR-151a-5p、miR-181a-5p、miR-185-5p、miR-194-5p、miR-199a-3p、miR-199b-3p、miR-212-3p、miR-363-3p、miR-548d-5p、miR-744-5p、miR-3117-3p、miR-5683和miR-5701——与心血管危险因素显著相关（双向相关系数>0.2，P值<0.006，错误发现率（FDR）<0.05）。特别值得关注的是，miR-5701与高血压、高胆固醇血症和糖尿病呈正相关。此外，我们发现miR-629-5p和miR-98-5p与急性心肌梗死显著相关。我们提供了CAD患者IMA样本中miR谱的首个图谱。从长远来看，这些miRs可能在改善CAD的风险评估、疾病机制理解和局部治疗中发挥重要作用。
