Facultad de Medicina Veterinaria y Zootecnia, Universidad de Colima, Kilometro 40 Autopista Colima-Manzanillo, Tecomán 28100, Colima, Mexico.
Departamento de Zootecnia, Posgrado en Producción Animal, Universidad Autónoma Chapingo, Km. 38.5 Carretera México-Texcoco, Chapingo 56230, Texcoco, Mexico.
Genes (Basel). 2021 Oct 22;12(11):1666. doi: 10.3390/genes12111666.
A genome-wide association study (GWAS) was performed to elucidate genetic architecture of growth traits in Braunvieh cattle. Methods: The study included 300 genotyped animals by the GeneSeek Genomic Profiler Bovine LDv.4 panel; after quality control, 22,734 SNP and 276 animals were maintained in the analysis. The examined phenotypic data considered birth (BW), weaning (WW), and yearling weights. The association analysis was performed using the principal components method via the egscore function of the GenABEL version 1.8-0 package in the R environment. The marker rs133262280 located in BTA 22 was associated with BW, and two SNPs were associated with WW, rs43668789 (BTA 11) and rs136155567 (BTA 27). New QTL associated with these liveweight traits and four positional and functional candidate genes potentially involved in variations of the analyzed traits were identified. The most important genes in these genomic regions were (minichromosome maintenance complex component 2), (transmembrane protein adipocyte associated 1), (), and (neuregulin 1), related to embryonic cleavage, bone and tissue growth, cell adhesion, and organic development. This study is the first to present a GWAS conducted in Braunvieh cattle in Mexico providing evidence for genetic architecture of assessed growth traits. Further specific analysis of found associated genes and regions will clarify its contribution to the genetic basis of growth-related traits.
一项全基因组关联研究（GWAS）旨在阐明夏洛莱牛生长性状的遗传结构。方法：本研究包括 300 头通过 GeneSeek Genomic Profiler Bovine LDv.4 面板进行基因分型的动物；经过质量控制后，在分析中保留了 22734 个 SNP 和 276 头动物。所检查的表型数据考虑了初生重（BW）、断奶重（WW）和 1 岁龄体重。关联分析使用主成分方法通过 GenABEL 版本 1.8-0 包中的 egscore 函数在 R 环境中进行。位于 BTA 22 的标记 rs133262280 与 BW 相关，两个 SNP 与 WW 相关，rs43668789（BTA 11）和 rs136155567（BTA 27）。鉴定出与这些活重性状相关的新 QTL 以及四个可能与分析性状变异相关的位置和功能候选基因。这些基因组区域中最重要的基因是 （微染色体维持复合物成分 2）、 （脂肪细胞相关跨膜蛋白 1）、 （）和 （神经调节素 1），与胚胎分裂、骨骼和组织生长、细胞黏附以及有机发育有关。本研究首次在墨西哥的夏洛莱牛中进行了 GWAS，为评估的生长性状的遗传结构提供了证据。对发现的相关基因和区域的进一步特异性分析将阐明其对与生长相关性状的遗传基础的贡献。
