牛基因启动子的克隆与转录活性分析：转录因子Sp1和GR调控牛的表达
Cloning and Transcriptional Activity Analysis of the Bovine Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine Expression.
作者信息
Liu Zhanxin, Bai Yanbin, Chen Zongchang, Niu Yanmei, Jia Xue, Li Liang, Zhang Xiaolan, Shi Binggang, Zhao Zhidong, Hu Jiang, Luo Yuzhu, Wang Jiqing, Liu Xiu, Li Shaobin, Zhao Fangfang
机构信息
Gansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
出版信息
Animals (Basel). 2025 Apr 25;15(9):1217. doi: 10.3390/ani15091217.
Growth rate of bovine skeletal muscle has a major impact on beef yield. Cadherin-11 () was found to be a potential candidate gene for growth and development in beef cattle. This study confirmed the high expression of in bovine using a tissue expression analysis. To understand the transcriptional regulation mechanism of gene, we constructed a double luciferase vector for the promoter region of gene and determined that the core transcriptional regulatory region was located at -129/+55 bp relative to the transcription start site (TSS). In addition, we confirmed that skeletal muscle growth and development-related transcription factor-specific protein 1 (SP1) and glucocorticoid receptor (GR) bind to the gene promoter region at -36/-27 bp and -20/-11 bp, respectively, to regulate expression. These interactions provide valuable information for understanding the mechanism of transcriptional regulation of the bovine gene during muscle growth and development.
牛骨骼肌的生长速度对牛肉产量有重大影响。钙黏蛋白-11（Cadherin-11）被发现是肉牛生长发育的一个潜在候选基因。本研究通过组织表达分析证实了Cadherin-11在牛肌肉中的高表达。为了解Cadherin-11基因的转录调控机制，我们构建了该基因启动子区域的双荧光素酶载体，并确定核心转录调控区域位于相对于转录起始位点（TSS）的-129/+55 bp处。此外，我们证实了与骨骼肌生长发育相关的转录因子特异性蛋白1（SP1）和糖皮质激素受体（GR）分别在-36/-27 bp和-20/-11 bp处与Cadherin-11基因启动子区域结合，以调节Cadherin-11的表达。这些相互作用为理解牛Cadherin-11基因在肌肉生长发育过程中的转录调控机制提供了有价值的信息。
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