Department of Microbiology, Culture Collection of Industrial Microorganisms-Microbiological Resource Center, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute, 02-532 Warsaw, Poland.
Department of Microbiology, Laboratory of Biotechnology and Molecular Engineering, Prof. Wacław Dąbrowski Institute of Agriculture and Food Biotechnology-State Research Institute, 02-532 Warsaw, Poland.
Genes (Basel). 2021 Oct 28;12(11):1720. doi: 10.3390/genes12111720.
Lactic acid bacteria (LAB) in the natural environment meet multiple stressors such as pH and temperature variations, increased nutrition and metabolite concentrations, harmful chemicals, acidic/oxidative conditions, osmotic pressure, and starvation. However, LAB strains are not subjected to high hydrostatic pressure (HHP) which currently is the most common non-thermal decontamination technology in the food industry. In this context, the LAB response to HHP is more difficult to identify compared to other stress-induced responses, and , , and can serve as essential regulators in this reaction. In the present study, the expression level of , , and mRNAs in 15 LAB strains after the HHP (300 MPa/5') exposure was evaluated. As a result, the HHP-treatment affected the up-regulation of , , and in KKP 3565, KKP 3566, KKP 3570, KKP 3575 strains, whereas, in KKP 3569, KKP 3571, KKP 3573 all genes were lower expressed. The relative expression level of the , , and either before or after the pressure treatment for DSM 6235, KKP 3572, KKP 3574, KKP 3576, KKP 3577, KKP 3578 strains were undetectable. Significant differences in the expression levels were observed, between the control and the HHP treatment strains for in KKP 3565, KKP 3566, KKP 3569, KKP 3570, KKP 3571, in, KKP 3565, KKP 3570, KKP 3571, and in KKP 3569, KKP 3571. Overall, the studied genes, , , and can be useful markers to indicate the LAB cellular response to HHP. These molecular parameters can help to optimize the desirable LAB growing conditions in industrial processes and to understand the complexity of the stress-related mechanism.
乳酸菌（LAB）在自然环境中会遇到多种胁迫因素，如 pH 值和温度变化、营养物质和代谢物浓度增加、有害物质、酸性/氧化条件、渗透压和饥饿等。然而，LAB 菌株不会受到高静水压（HHP）的影响，HHP 目前是食品工业中最常见的非热消毒技术。在这种情况下，与其他应激诱导反应相比，LAB 对 HHP 的反应更难识别，而 、 、 和 可以作为该反应中的重要调节剂。在本研究中，评估了 15 株 LAB 菌株在 HHP（300 MPa/5'）暴露后的 、 、 和 mRNAs 的表达水平。结果表明，HHP 处理影响了 、 、 和 在 KKP 3565、KKP 3566、KKP 3570、KKP 3575 菌株中的上调，而在 KKP 3569、KKP 3571、KKP 3573 中所有基因的表达水平均较低。在压力处理前后， 、 、 和 的相对表达水平对于 DSM 6235、KKP 3572、KKP 3574、KKP 3576、KKP 3577、KKP 3578 菌株均不可检测。在 KKP 3565、KKP 3566、KKP 3569、KKP 3570、KKP 3571、 、 、 、 、KKP 3569、KKP 3571 中，在 KKP 3565、KKP 3570、KKP 3571 中，在 KKP 3569、KKP 3571 中， 、 、 和 基因的表达水平在对照和 HHP 处理菌株之间存在显著差异。总之，研究的基因 、 、 和 可以作为指示 LAB 细胞对 HHP 反应的有用标记。这些分子参数可以帮助优化工业过程中理想的 LAB 生长条件，并理解与应激相关的机制的复杂性。
