División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí 78216, Mexico.
Centro Nacional de Supercómputo, Instituto Potosino de Investigación Científica y Tecnológica A.C., Consejo Nacional de Ciencia y Tecnología, San Luis Potosí 78216, Mexico.
Genes (Basel). 2021 Oct 29;12(11):1745. doi: 10.3390/genes12111745.
Tomato is one of the most important crops for human consumption. Its production is affected by the actinomycete subsp. (), one of the most devastating bacterial pathogens of this crop. Several wild tomato species represent a source of natural resistance to . Here, we contrasted the transcriptomes of the resistant wild tomato species LA2157 and the susceptible species cv. Ailsa Craig, during the first 24 h of challenge with . We used three analyses approaches which demonstrated to be complementary: mapping to reference genome SL3.0; semi de novo transcriptome assembly; and de novo transcriptome assembly. In a global context, transcriptional changes seem to be similar between both species, although there are some specific genes only upregulated in during interaction, suggesting that the resistance regulatory mechanism probably diverged during the domestication process. Although showed enriched functional groups related to defense, displayed a higher number of induced genes related to bacterial, oomycete, and fungal defense at the first few hours of interaction. This study revealed genes that may contribute to the resistance phenotype in the wild tomato species, such as those that encode for a polyphenol oxidase E, diacyl glycerol kinase, TOM1-like protein 6, and an ankyrin repeat-containing protein, among others. This work will contribute to a better understanding of the defense mechanism against , and the development of new control methods.
番茄是人类消费的最重要作物之一。其生产受到放线菌亚属（）的影响，这是该作物最具破坏性的细菌病原体之一。几种野生番茄物种代表了对的天然抗性的来源。在这里，我们在接种后的前 24 小时内对比了抗感野生番茄物种 LA2157 和栽培品种 Ailsa Craig 的转录组。我们使用了三种互补的分析方法：映射到参考基因组 SL3.0；半从头转录组组装；和从头转录组组装。在全局范围内，两种物种之间的转录变化似乎相似，尽管有些特定基因仅在与相互作用时上调，表明抗性调控机制可能在驯化过程中发生了分歧。尽管显示出与防御相关的功能富集，但在相互作用的最初几个小时内，显示出更多与细菌、卵菌和真菌防御相关的诱导基因。这项研究揭示了可能有助于野生番茄物种抗性表型的基因，例如编码多酚氧化酶 E、二酰基甘油激酶、TOM1 样蛋白 6 和富含锚蛋白重复的蛋白等基因。这项工作将有助于更好地理解针对的防御机制，并开发新的控制方法。
