利用微量唾液进行SARS-CoV-2病毒快速诊断技术的可行性

Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva.

作者信息

Tokuyama-Toda Reiko, Muraoka Masaaki, Terada-Ito Chika, Ide Shinji, Horiuchi Toshikatsu, Amemiya Tsuyoshi, Fukuoka Airi, Hamada Yoshiki, Sejima Shunsuke, Satomura Kazuhito

机构信息

Department of Oral Medicine and Stomatology, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama City 230-8501, Japan.

Certified Non-Profit Organization Biomedical Science Association, 2-20-8, Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan.

出版信息

Diagnostics (Basel). 2021 Nov 1;11(11):2024. doi: 10.3390/diagnostics11112024.

DOI:10.3390/diagnostics11112024

PMID:34829371

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8625231/

Abstract

Containment of SARS-CoV-2 has become an urgent global issue. To overcome the problems of conventional quantitative polymerase chain reaction (qPCR) tests, we verified the usefulness of a mobile qPCR device that utilizes mouthwash to obtain a saliva sample with the aim of developing a rapid diagnostic method for SARS-CoV-2. First, we examined whether anyone could easily operate this device. Then, we examined whether RNA in the mouthwash could be detected in a short time. In addition, we investigated whether it was possible to diagnose SARS-CoV-2 infection using mouthwash obtained from COVID-19 patients undergoing hospitalization. The results revealed that all subjects were able to complete the operation properly without error. In addition, RNase P was detected in the mouthwash without pretreatment. The average detection time was 18 min, which is significantly shorter than conventional qPCR devices. Furthermore, this device detected SARS-CoV-2 in the mouthwash of a COVID-19 patient undergoing hospitalization. The above findings verified the efficacy of this diagnostic method, which had a low risk of infection, was technically simple, and provided stable results. Therefore, this method is useful for the rapid detection of SARS-CoV-2.

摘要

新型冠状病毒（SARS-CoV-2）的防控已成为紧迫的全球问题。为克服传统定量聚合酶链反应（qPCR）检测的问题，我们验证了一种移动qPCR设备的实用性，该设备利用漱口水获取唾液样本，旨在开发一种针对SARS-CoV-2的快速诊断方法。首先，我们检查了任何人是否都能轻松操作该设备。然后，我们检查了漱口中的RNA能否在短时间内被检测到。此外，我们研究了使用从住院的COVID-19患者那里获取的漱口水来诊断SARS-CoV-2感染是否可行。结果显示，所有受试者均能正确无误地完成操作。此外，未经预处理的漱口中检测到了核糖核酸酶P（RNase P）。平均检测时间为18分钟，明显短于传统qPCR设备。此外，该设备在一名住院的COVID-19患者的漱口中检测到了SARS-CoV-2。上述发现证实了这种诊断方法的有效性，其感染风险低、技术操作简单且结果稳定。因此，该方法有助于快速检测SARS-CoV-2。