Shokr Hala, Dias Irundika Hk, Gherghel Doina
Vascular Research Laboratory, College of Health and Life Sciences, Aston University, Birmingham B4 7ET, UK.
Aston Medical Research Institute, Aston Medical School, Aston University, Birmingham B4 7ET, UK.
Antioxidants (Basel). 2021 Nov 3;10(11):1756. doi: 10.3390/antiox10111756.
The aim of the present paper is to assess the relationship between oxysterol levels and retinal microvascular function in individuals of various age groups, free of clinically evident diseases. Forty-two apparently healthy individuals were included in the present study (group 1: 19-30 years, group 2: 31-50 years, and group 3: 51-70 years). Retinal microvascular function was assessed using the dynamic retinal vessel analyzer (DVA, IMEDOS GmbH, Jena, Germany). Fasting plasma was obtained from all subjects and quantification of monohydroxy and dihydroxy oxysterols assessment was performed using LC-MS/MS following reverse phase chromatography. A Griess assay was used to evaluate the Nitric Oxide (NO) concentration in all individuals. The glutathione redox ratio was also analyzed by means of whole blood glutathione recycling assay. In all participants, the levels of 7-Ketocholesterol, 25-hydroxycholesterol and 7β-hydroxycholesterol correlated significantly and positively with the time to maximum arteriolar dilation. In addition, 25-hydroxycholesterol and 7β-hydroxycholesterol negatively correlated to the percentage of maximum arteriolar dilation. A negative correlation was observed for 27-hydroxycholesterol and 7β-hydroxycholesterol with microvascular arteriolar constriction. These results suggest that, with age, abnormal oxysterol levels correlate with early changes in microvascular bed function. This relationship could signal early risk for cardiovascular diseases (CVDs) in an ageing population.
本文旨在评估不同年龄组、无临床明显疾病个体的氧化甾醇水平与视网膜微血管功能之间的关系。本研究纳入了42名明显健康的个体（第1组：19 - 30岁，第2组：31 - 50岁，第3组：51 - 70岁）。使用动态视网膜血管分析仪（DVA，德国耶拿IMEDOS GmbH公司）评估视网膜微血管功能。从所有受试者获取空腹血浆，并采用反相色谱法结合LC - MS/MS对单羟基和二羟基氧化甾醇进行定量评估。使用格里斯试剂法评估所有个体的一氧化氮（NO）浓度。还通过全血谷胱甘肽循环测定法分析谷胱甘肽氧化还原比。在所有参与者中，7 - 酮胆固醇、25 - 羟基胆固醇和7β - 羟基胆固醇的水平与最大小动脉扩张时间呈显著正相关。此外，25 - 羟基胆固醇和7β - 羟基胆固醇与最大小动脉扩张百分比呈负相关。观察到27 - 羟基胆固醇和7β - 羟基胆固醇与微血管小动脉收缩呈负相关。这些结果表明，随着年龄增长，氧化甾醇水平异常与微血管床功能的早期变化相关。这种关系可能预示着老年人群心血管疾病（CVD）的早期风险。
