Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, Leeds LS9 7TF, UK.
Novogene Europe, 25 Science Park, Milton, Cambridge CB4 0FW, UK.
Int J Mol Sci. 2021 Nov 12;22(22):12225. doi: 10.3390/ijms222212225.
The aging of bone marrow (BM) remains a very imperative and alluring subject, with an ever-increasing interest among fellow scientists. A considerable amount of progress has been made in this field with the established 'hallmarks of aging' and continued efforts to investigate the age-related changes observed within the BM. Inflammaging is considered as a low-grade state of inflammation associated with aging, and whilst the possible mechanisms by which aging occurs are now largely understood, the processes leading to the underlying changes within aged BM remain elusive. The ability to identify these changes and detect such alterations at the genetic level are key to broadening the knowledgebase of aging BM. Next-generation sequencing (NGS) is an important molecular-level application presenting the ability to not only determine genomic base changes but provide transcriptional profiling (RNA-seq), as well as a high-throughput analysis of DNA-protein interactions (ChIP-seq). Utilising NGS to explore the genetic alterations occurring over the aging process within alterative cell types facilitates the comprehension of the molecular and cellular changes influencing the dynamics of aging BM. Thus, this review prospects the current landscape of BM aging and explores how NGS technology is currently being applied within this ever-expanding field of research.
骨髓（BM）的衰老仍然是一个非常重要和吸引人的课题，越来越多的科学家对此产生了兴趣。随着“衰老的特征”的确立以及对 BM 中观察到的与年龄相关的变化的持续研究，该领域取得了相当大的进展。炎衰老被认为是与衰老相关的低度炎症状态，虽然现在已经在很大程度上了解了衰老发生的可能机制，但导致衰老 BM 内部潜在变化的过程仍然难以捉摸。识别这些变化并在遗传水平上检测到这些改变是拓宽衰老 BM 知识库的关键。下一代测序（NGS）是一个重要的分子水平应用，它不仅能够确定基因组碱基变化，还能够提供转录谱分析（RNA-seq），以及 DNA-蛋白质相互作用的高通量分析（ChIP-seq）。利用 NGS 来探索在替代性细胞类型中随着衰老过程而发生的遗传改变，有助于理解影响衰老 BM 动态的分子和细胞变化。因此，本综述展望了 BM 衰老的现状，并探讨了 NGS 技术在这一不断扩展的研究领域中的应用。
