Kasama Toshihiro, Sun Miaomiao, Kaji Noritada, Akiyama Shin'ichi, Yuzawa Yukio, Tokeshi Manabu, Matsuo Seiichi, Baba Yoshinobu
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan.
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Aichi 464-8603, Japan.
Micromachines (Basel). 2021 Oct 31;12(11):1353. doi: 10.3390/mi12111353.
This study developed low-cost and highly sensitive immunoassay devices possessing the ability to rapidly analyze urine samples. Further, they can quantitatively detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), and liver-type fatty acid binding protein (L-FABP). The devices were used to successfully estimate the concentrations of the three biomarkers in urine samples within 2 min; the results were consistent with those obtained via conventional enzyme-linked immunosorbent assay (ELISA), which requires several hours. In addition, the estimated detection limits for the three biomarkers were comparable to those of commercially available ELISA kits. Thus, the proposed and fabricated devices facilitate high-precision and frequent monitoring of renal function.
本研究开发了具有快速分析尿液样本能力的低成本、高灵敏度免疫分析装置。此外，它们能够定量检测三种指示肾损伤的生物标志物：单核细胞趋化蛋白1（MCP-1）、血管紧张素原（AGT）和肝型脂肪酸结合蛋白（L-FABP）。这些装置用于在2分钟内成功估算尿液样本中三种生物标志物的浓度；结果与通过传统酶联免疫吸附测定（ELISA）获得的结果一致，而传统ELISA需要数小时。此外，三种生物标志物的估计检测限与市售ELISA试剂盒相当。因此，所提出并制造的装置有助于对肾功能进行高精度和频繁的监测。
