Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Lab Chip. 2010 Dec 21;10(24):3335-40. doi: 10.1039/c0lc00241k. Epub 2010 Oct 19.
We present a new rapid and easy-to-use immunoassay chip which we have named the immuno-pillar chip. It has hydrogel pillars, fabricated inside a microchannel, with many antibody molecules immobilized onto 1 µm diameter polystyrene beads. To evaluate the chip performance, we applied it to the sandwich assay of C-reactive protein (CRP), α-fetoprotein (AFP) and prostate-specific antigen (PSA), a cardiac and inflammation marker, tumors and prostate cancer markers, respectively. For detection of disease markers, we confirmed the chip provides rapid analysis (total assay time of about 4 min) with high sensitivity, it is easy-to-use (no special skills are needed), and it uses small volumes of the sample and reagent (0.25 µL each). Moreover, multiplex assay for three biomarkers was also possible. Additionally, the immuno-pillar chip has a big advantage of having hardly any influence on the assay results even if the introduction quantities of the sample or reagents are different.
我们提出了一种新的快速且易于使用的免疫分析芯片，我们将其命名为免疫柱芯片。它具有水凝胶柱，在微通道内部制造，许多抗体分子固定在 1 µm 直径的聚苯乙烯珠上。为了评估芯片的性能，我们将其应用于 C 反应蛋白（CRP）、甲胎蛋白（AFP）和前列腺特异性抗原（PSA）的夹心检测，分别是心脏和炎症标志物、肿瘤和前列腺癌标志物。为了检测疾病标志物，我们证实该芯片提供了快速分析（总分析时间约为 4 分钟），具有高灵敏度，易于使用（不需要特殊技能），并且使用小体积的样品和试剂（每个 0.25 µL）。此外，还可以进行三种生物标志物的多重分析。此外，即使样品或试剂的引入量不同，免疫柱芯片几乎对分析结果没有影响，这是它的一个很大的优势。
