College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China.
Key Laboratory of Effective Components of Traditional Chinese Medicine, Changchun 130117, China.
Molecules. 2022 Oct 26;27(21):7253. doi: 10.3390/molecules27217253.
Fourteen compounds were isolated from (Willd.) Ohwi by column chromatography and preparative thin-layer chromatography; the structures were identified by spectroscopic analysis and compared with data reported in the literature. Seven compounds were isolated and identified from for the first time: Linoleic acid, Sandwicensin, Isovanillin, Ethyl ferulate, Haginin A, Isopterofuran, 3'.7-Dihydroxyisoflavan. The other 10 compounds were structurally identified as follows: Lupenone, Lupeol, β-sitosterol, Genistein, Medicarpin, Coniferyl Aldehyde, Syringaldehyde. All compounds were evaluated for their ability to inhibit SW480 and SW620 cells using the CCK-8 method; compound (Sandwicensin) had the best activity, and compounds , , and exhibited moderate inhibitory activity. In addition, the targets and signaling pathways of Sandwicensin treatment for CRC were mined using network pharmacology, and MAPK3, MTOR, CCND1 and CDK4 were found to be closely associated with Sandwicensin treatment for CRC; the GO and KEGG analysis showed that Sandwicensin may directly regulate the cycle, proliferation and apoptosis of CRC cells through cancer-related pathways.
从 （Willd.）Ohwi 中通过柱层析和制备薄层层析分离得到 14 种化合物；通过光谱分析鉴定了它们的结构，并与文献报道的数据进行了比较。首次从 中分离鉴定出 7 种化合物：亚油酸、 sandwicensin、异香草醛、阿魏酸乙酯、haginina A、异阿魏呋喃、3'.7-二羟基异黄酮。其他 10 种化合物的结构鉴定如下：羽扇豆酮、羽扇豆醇、β-谷甾醇、染料木素、芒柄花素、松柏醛、丁香醛。所有化合物均采用 CCK-8 法评价其对 SW480 和 SW620 细胞的抑制作用；化合物 （sandwicensin）活性最好，化合物 、 、 、 表现出中等抑制活性。此外，还利用网络药理学对 sandwicensin 治疗 CRC 的靶点和信号通路进行了挖掘，发现 MAPK3、MTOR、CCND1 和 CDK4 与 sandwicensin 治疗 CRC 密切相关；GO 和 KEGG 分析表明，sandwicensin 可能通过与癌症相关的途径直接调节 CRC 细胞的周期、增殖和凋亡。
