Marin Nicoleta Mirela, Stanculescu Ioana
National Research and Development Institute for Industrial Ecology ECOIND, Street Podu Dambovitei No. 57-73, District 6, 060652 Bucharest, Romania.
Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Bd., 030018 Bucharest, Romania.
Polymers (Basel). 2021 Nov 18;13(22):3991. doi: 10.3390/polym13223991.
Despite Acid Blue 113 (AB 113)'s extensive use and negative environmental impact, very few studies have focused on its efficient and environmentally friendly removal. This research aims the removal of AB 113 from environmental aqueous media and its consequent enzymatic biodegradation. A strongly basic anion exchange resin in Cl form, Amberlite IRA 402 (IRA 402(Cl)) was used for AB 113 adsorption and a laccase was used to further biodegrade it. For the first time, two novel, efficient and environmentally friendly physical-chemical and biological assays for AB 113 wastewater removal and subsequent biodegradation were combined. The adsorption of AB 113 onto IRA 402(Cl) was tested in batch and continuous flux modes. Influence of contact time, concentration and desorption in acidic media were evaluated. The kinetic data were best modulated by the Lagergren model with R = 0.9275. The Langmuir isotherm model best fitted the experimental data, and the maximum adsorption capacity was 130 mg/g. Dye, resin and AB113 loaded resin were characterized by thermogravimetry and FTIR to evaluate their physical chemical properties modification. Based on the performed studies, a consecutive methodology is proposed, incorporating the ion exchange process in the first stage and the biodegradation process in the second. Thus, in the second stage the residual concentration of AB 113 is reduced by an efficient bio-degradation process produced by the laccase at pH = 4.
尽管酸性蓝113（AB 113）被广泛使用且对环境有负面影响，但很少有研究关注其高效且环保的去除方法。本研究旨在从环境水介质中去除AB 113及其后续的酶促生物降解。采用Cl型强碱性阴离子交换树脂Amberlite IRA 402（IRA 402(Cl)）对AB 113进行吸附，并使用漆酶对其进一步生物降解。首次将两种新颖、高效且环保的物理化学和生物方法相结合，用于去除AB 113废水并进行后续生物降解。以分批和连续通量模式测试了AB 113在IRA 402(Cl)上的吸附情况。评估了接触时间、浓度和酸性介质中解吸的影响。动力学数据最适合用Lagergren模型进行拟合，R = 0.9275。Langmuir等温线模型最符合实验数据，最大吸附容量为130 mg/g。通过热重分析和傅里叶变换红外光谱对染料、树脂和负载AB113的树脂进行了表征，以评估它们物理化学性质的变化。基于所进行的研究，提出了一种连续的方法，第一阶段采用离子交换过程，第二阶段采用生物降解过程。因此，在第二阶段，通过漆酶在pH = 4时产生的高效生物降解过程降低了AB 113的残留浓度。
