谷氨酸脱氢酶在谷氨酸代谢和 中的应激抗性中的作用。
Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance in .
机构信息
CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
出版信息
Molecules. 2021 Nov 10;26(22):6793. doi: 10.3390/molecules26226793.
is an important laver species in China. Its quality traits are closely related to the content of glutamic acid. Glutamate dehydrogenase (GDH) is a crucial enzyme in the glutamic acid metabolism. In this study, two genes from , 1 and 2, were cloned and successfully expressed in . The in vitro enzyme activity assay demonstrated that the catalytic activity of PhGDHs is mainly in the direction of ammonium assimilation. The measured values of PhGDH1 for NADH, (NH)SO, and α-oxoglutarate were 0.12, 4.99, and 0.16 mM, respectively, while the corresponding values of PhGDH2 were 0.02, 3.98, and 0.104 mM, respectively. Site-directed mutagenesis results showed that Gly and Thr were important catalytic residues for PhGDH2. Moreover, expression levels of both PhGDHs were significantly increased under abiotic stresses. These results suggest that PhGDHs can convert α-oxoglutarate to glutamic acid, and enhance the flavor and stress resistance of .
是中国重要的贻贝品种。其品质特性与谷氨酸含量密切相关。谷氨酸脱氢酶（GDH）是谷氨酸代谢中的关键酶。本研究从 克隆并成功表达了两个基因，1 和 2。体外酶活性测定表明，PhGDHs 的催化活性主要在铵同化方向。PhGDH1 对 NADH、(NH)SO 和 α-酮戊二酸的 值分别为 0.12、4.99 和 0.16 mM，而 PhGDH2 的相应 值分别为 0.02、3.98 和 0.104 mM。定点突变结果表明 Gly 和 Thr 是 PhGDH2 的重要催化残基。此外，两种 PhGDHs 的表达水平在非生物胁迫下均显著增加。这些结果表明 PhGDHs 可以将 α-酮戊二酸转化为谷氨酸，从而提高 的风味和抗逆性。
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