Al-Dakhil Mohammed, Alghamdi Salem, Migdadi Hussein, Afzal Muhammad, Ali Ahmed Abdelrahim
Natural Resources and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2021 Nov 12;10(11):2438. doi: 10.3390/plants10112438.
Duckweeds, or Lemnaceae, are widespread aquatic plants. Morphology-based identification of duckweed species is difficult because of their structural complexity. Hence, molecular tools provide significant advantages for characterizing and selecting species or clones for sustainable commercial use. In this study, we collected and characterized ten duckweed isolates from nine different regions in Saudi Arabia (SA). Based on the morphological characterization and phylogenetic analysis of intergenic spacer sequences of chloroplast DNA using six barcoding markers, the clones were classified into three genera, represented by seven species: L., L., Landolt, Welw., Torr., (L.) Schleid., and G. Mey. was revealed to be a distinct dominant duckweed species in many regions of SA. Five barcoding markers showed that , , and were the most widely distributed species in the country. However, , and were the dominant species in the Al-Qassim, Madinah-1, and Madinah-2 regions, respectively. Moreover, the morphological traits revealed variations for these clones, relative to other studied duckweed clones. According to the results obtained in this study, three out of six plastid markers (H-A, , and F-H) helped to identify the dominant duckweed species in Saudi Arabia. Further evaluation based on adaptability, molecular genetic studies, and functional genomics is needed for these species to be used at the commercial level in Saudi Arabia.
浮萍，即浮萍科植物，是广泛分布的水生植物。由于浮萍结构复杂，基于形态学对其进行物种鉴定具有难度。因此，分子工具为可持续商业用途的物种特征描述和选择提供了显著优势。在本研究中，我们收集并鉴定了来自沙特阿拉伯（SA）九个不同地区的十种浮萍分离株。基于形态学特征以及使用六个条形码标记对叶绿体DNA基因间隔序列进行的系统发育分析，这些克隆被分为三个属，由七个物种代表：L.、L.、Landolt、Welw.、Torr.、(L.) Schleid.和G. Mey。在沙特阿拉伯的许多地区，被揭示为一种独特的优势浮萍物种。五个条形码标记显示，、和是该国分布最广泛的物种。然而，、和分别是卡西姆、麦地那 - 1和麦地那 - 2地区的优势物种。此外，相对于其他研究的浮萍克隆，形态特征揭示了这些克隆的变异。根据本研究获得的结果，六个质体标记中的三个（H - A、和F - H）有助于鉴定沙特阿拉伯的优势浮萍物种。要在沙特阿拉伯将这些物种用于商业层面，还需要基于适应性、分子遗传学研究和功能基因组学进行进一步评估。
