Molecular Immunovirology Laboratory, General Biology Department, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil.
Microbiology Department, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil.
Viruses. 2021 Oct 21;13(11):2123. doi: 10.3390/v13112123.
Mayaro virus (MAYV) is a neglected arthropod-borne virus found in the Americas. MAYV infection results in Mayaro fever, a non-lethal debilitating disease characterized by a strong inflammatory response affecting the joints and muscles. MAYV was once considered endemic to forested areas in Brazil but has managed to adapt and spread to urban regions using new vectors, such as , and has the potential to cause serious epidemics in the future. Currently, there are no vaccines or specific treatments against MAYV. In this study, the antiviral activity of a series of synthetic cyclic ketones were evaluated for the first time against MAYV. Twenty-four compounds were screened in a cell viability assay, and eight were selected for further evaluation. Effective concentration (EC) and selectivity index (SI) were calculated and compound 9-(5-(4-chlorophenyl]furan-2-yl)-3,6-dimethyl-3,4,5,6,7,9-hexahydro-1H-xanthene-1,8(2))-dione (9) (EC = 21.5 µmol·L, SI = 15.8) was selected for mechanism of action assays. The substance was able to reduce viral activity by approximately 70% in both pre-treatment and post-treatment assays.
马亚罗病毒（MAYV）是一种被忽视的节肢动物传播病毒，存在于美洲。MAYV 感染会导致马亚罗热，这是一种非致命性的使人虚弱的疾病，其特征是强烈的炎症反应，影响关节和肌肉。MAYV 曾被认为是巴西森林地区的地方病，但已成功适应并通过新的媒介传播到城市地区，如 ，并有潜力在未来引发严重的流行病。目前，尚无针对 MAYV 的疫苗或特定治疗方法。在这项研究中，首次评估了一系列合成环酮对 MAYV 的抗病毒活性。在细胞活力测定中筛选了 24 种化合物，选择了 8 种进行进一步评估。计算了有效浓度（EC）和选择性指数（SI），并选择了 9-(5-(4-氯苯基）呋喃-2-基）-3,6-二甲基-3,4,5,6,7,9-六氢-1H-呫吨-1,8(2))-二酮（9）（EC = 21.5 μmol·L，SI = 15.8）进行作用机制检测。该物质在预处理和后处理试验中均可使病毒活性降低约 70%。
