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白果酚酸对基孔肯雅热、马亚罗热、乌纳热和 Zika 病毒的广谱抗病毒活性。

Broad Antiviral Activity of Ginkgolic Acid against Chikungunya, Mayaro, Una, and Zika Viruses.

机构信息

Grupo de Biología Celular y Molecular de Arbovirus, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá 0816-02593, Panamá.

Programa de Doctorado en Ciencias Biológicas, Universidad de la República, Montevideo 11200, Uruguay.

出版信息

Viruses. 2020 Apr 15;12(4):449. doi: 10.3390/v12040449.

DOI:10.3390/v12040449
PMID:32326564
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7232212/
Abstract

The alphaviruses Chikungunya (CHIKV), Mayaro (MAYV), Una (UNAV), and the flavivirus Zika (ZIKV) are emerging or re-emerging arboviruses which are responsible for frequent epidemic outbreaks. Despite the large impact of these arboviruses on health systems, there are no approved vaccines or treatments to fight these infections. As a consequence, there is an urgent need to discover new antiviral drugs. Natural products are a rich source of compounds with distinct biological activities, including antiviral properties. Thus, we aimed to explore the potential antiviral activity of Ginkgolic acid against the arboviruses CHIKV, MAYV, UNAV, and ZIKV. Viral progeny production in supernatants from cells treated or not treated with Ginkgolic acid was quantified by plaque-forming assay. Ginkgolic acid's direct virucidal activity against these arboviruses was also determined. Additionally, viral protein expression was assessed using Western blot and immunofluorescence. Our results reveal that Ginkgolic acid promotes a dose-dependent decrease in viral titers in all tested viruses. Moreover, the compound demonstrated strong virucidal activity. Finally, we found that viral protein expression was affected by treatment with this drug. Collectively, these findings suggest that Ginkgolic acid could have broader antiviral activity.

摘要

甲型病毒中的基孔肯雅热病毒（CHIKV）、马亚罗病毒（MAYV）、乌纳病毒（UNAV）和黄病毒中的 Zika 病毒（ZIKV）是新兴或重现的虫媒病毒，它们是导致频繁爆发的流行病的罪魁祸首。尽管这些虫媒病毒对卫生系统造成了巨大影响，但目前还没有针对这些感染的批准疫苗或治疗方法。因此，迫切需要发现新的抗病毒药物。天然产物是具有独特生物活性的化合物的丰富来源，包括抗病毒特性。因此，我们旨在探索白果酚酸对虫媒病毒 CHIKV、MAYV、UNAV 和 ZIKV 的潜在抗病毒活性。通过噬斑形成测定法来定量测定用或未用白果酚酸处理的细胞上清液中的病毒后代产生情况。还确定了白果酚酸对这些虫媒病毒的直接病毒杀灭活性。此外，还使用 Western blot 和免疫荧光法评估了病毒蛋白的表达。我们的研究结果表明，白果酚酸可促进所有测试病毒的病毒滴度呈剂量依赖性下降。此外，该化合物表现出很强的病毒杀灭活性。最后，我们发现该药物处理会影响病毒蛋白的表达。总的来说，这些发现表明白果酚酸可能具有更广泛的抗病毒活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/fcca6393385d/viruses-12-00449-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/4a7f6e34e653/viruses-12-00449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/fdb98072c1f4/viruses-12-00449-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/71a9f82e6eca/viruses-12-00449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/5ed8db1ab8cc/viruses-12-00449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/8d3b885d205d/viruses-12-00449-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/fcca6393385d/viruses-12-00449-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/4a7f6e34e653/viruses-12-00449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/fdb98072c1f4/viruses-12-00449-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/71a9f82e6eca/viruses-12-00449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/5ed8db1ab8cc/viruses-12-00449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/8d3b885d205d/viruses-12-00449-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d97f/7232212/fcca6393385d/viruses-12-00449-g006.jpg

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