The Peter Doherty Institute of Immunity and Infection, Department of Microbiology and Immunity, University of Melbourne, Melbourne, VIC 3000, Australia.
Sydney Institute for Infectious Diseases, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.
Viruses. 2021 Oct 21;13(11):2122. doi: 10.3390/v13112122.
Metagenomic next-generation sequencing has transformed the discovery and diagnosis of infectious disease, with the power to characterise the complete 'infectome' (bacteria, viruses, fungi, parasites) of an individual host organism. However, the identification of novel pathogens has been complicated by widespread microbial contamination in commonly used laboratory reagents. Using total RNA sequencing ("metatranscriptomics") we documented the presence of contaminant viral sequences in multiple 'blank' negative control sequencing libraries that comprise a sterile water and reagent mix. Accordingly, we identified 14 viral sequences in 7 negative control sequencing libraries. As in previous studies, several circular replication-associated protein encoding (CRESS) DNA virus-like sequences were recovered in the blank control libraries, as well as contaminating sequences from the , and families of RNA virus. These data suggest that viral contamination of common laboratory reagents is likely commonplace and can comprise a wide variety of viruses.
宏基因组下一代测序技术改变了传染病的发现和诊断方式，使我们能够描述个体宿主生物的完整“传染组”（细菌、病毒、真菌、寄生虫）。然而，由于常见实验室试剂中广泛存在微生物污染，新病原体的鉴定变得复杂起来。通过对总 RNA 测序（“宏转录组学”）的研究，我们在由无菌水和试剂混合组成的多个“空白”阴性对照测序文库中记录了污染病毒序列的存在。因此，我们在 7 个阴性对照测序文库中鉴定出了 14 种病毒序列。与之前的研究一样，在空白对照文库中回收了多个环状复制相关蛋白编码（CRESS）DNA 病毒样序列，以及来自 、 和 家族的 RNA 病毒的污染序列。这些数据表明，常见实验室试剂中的病毒污染可能很常见，并且可能包含多种病毒。
