Applied Molecular Biology Lab-LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59064-720, Brazil.
Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal 59064-720, Brazil.
Viruses. 2021 Oct 26;13(11):2160. doi: 10.3390/v13112160.
2A is an oligopeptide sequence that mediates a ribosome "skipping" effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.
2A 是一个寡肽序列，介导核糖体“跳跃”效应，并能介导多蛋白的共翻译切割。这些序列广泛分布于从昆虫到哺乳动物的病毒中，可能通过加速适应性发挥作用。这些序列已被用于许多异源共表达系统，因为它们是切割具有生物技术意义的蛋白质的多功能工具。在这项工作中，我们通过筛选国家生物技术信息中心数据库中可用的序列，回顾和更新了不同病毒群中 2A/2A 样序列的发生情况。有趣的是，我们首次报道了 69 个序列中存在 2A 样序列。其中，62 个对应于正单链 RNA 物种，6 个对应于双链 RNA 病毒，1 个对应于负单链 RNA 病毒。还讨论了这些序列对病毒进化的重要性及其在生物技术应用中的潜力。
