通过快速涨落增强结构光照明显微镜观察酵母Septins的动态结构
Dynamic Structure of Yeast Septin by Fast Fluctuation-Enhanced Structured Illumination Microscopy.
作者信息
Yao Longfang, Zhang Li, Chen Liwen, Gong Xingyu, Zhong Jiahui, Wang Baoju, Fei Yiyan, Mi Lan, Ma Jiong
机构信息
Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China.
Shanghai Engineering Research Center of Industrial Microorganisms, The Multiscale Research Institute of Complex Systems (MRICS), School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
出版信息
Microorganisms. 2021 Oct 29;9(11):2255. doi: 10.3390/microorganisms9112255.
When divides, a structure composed of different septin proteins arranged according to a certain rule is formed at the cell division site. The structure undergoes multiple remodeling stages during the cell cycle, thus guiding the yeast cells to complete the entire division process. Although the higher-order structure of septins can be determined using electron microscopy, the septin's dynamic processes are poorly understood because of limitations in living cell super-resolution imaging technology. Herein, we describe a high lateral resolution and temporal resolution technique, known as fast fluctuation-enhanced structured illumination microscopy (fFE-SIM), which more than doubles the SIM resolution at a frame rate of 38 Hz in living cells. This allows a highly dynamic and sparse septin structure to be observed in .
当细胞分裂时，在细胞分裂位点会形成一种由不同的septin蛋白按照一定规则排列组成的结构。该结构在细胞周期中经历多个重塑阶段，从而引导酵母细胞完成整个分裂过程。尽管可以使用电子显微镜确定septin的高阶结构，但由于活细胞超分辨率成像技术的局限性，septin的动态过程仍知之甚少。在此，我们描述了一种具有高横向分辨率和时间分辨率的技术，称为快速波动增强结构光照明显微镜（fFE-SIM），它在活细胞中以38Hz的帧率使SIM分辨率提高了一倍多。这使得能够在……中观察到高度动态且稀疏的septin结构。
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