Sun Xiao-Ge, Wang Yue, Xie Tian, Yang Zhan-Tao, Wang Ji-Dong, Zheng Yu-Hui, Guo Cheng, Zhang Yan, Wang Qian-Qian, Wang Zhong-Han, Wang Wei, Wang Ya-Jing, Yang Hong-Jian, Li Sheng-Li
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Animal Production Systems Group, Wageningen University & Research, 6708PB Wageningen, The Netherlands.
Microorganisms. 2021 Nov 5;9(11):2304. doi: 10.3390/microorganisms9112304.
Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage diets on in vitro ruminal fermentation, gas composition, and microbial profile. Three isoenergetic and isoproteic experimental diets were designed and used as fermentation substrate: control treatment (CON group) was the basal diet lacking oilseeds, the other two treatments were the basal diet supplemented by 100 g/kg dry matter (DM) raw whole soybean (S group) and 50 g/kg DM raw flaxseed (F group), respectively. Data showed that the acetate, butyrate, and total VFA concentration of culture fluids in the S group were lower ( < 0.05) than in the F group. There was a tendency to a higher level ( = 0.094) of propionate concentration in the F group compared with the other two groups. The gas production in the F group was higher ( < 0.05) than in the control group. There was a lower abundance of ( < 0.05) and a greater abundance of ( < 0.05) in both of the two oilseed treatments. ( = 0.078) in the F group was the lowest. Our results suggested that CH emission could be inhibited with flaxseed supplementation by propionate production metabolism, biohydrogenation of unsaturated fatty acid (FA), and toxicity to , while regarding soybean seed supplementation, the emission of CH was more likely to be reduced through biohydrogenation of unsaturated FA modulated by .
脂质代谢在反刍动物的能量代谢中起着重要作用。然而，其脂肪、瘤胃发酵、气体排放和微生物之间的相互作用尚不清楚。本研究评估了在高粗饲料日粮中添加生油籽对体外瘤胃发酵、气体成分和微生物群落的影响。设计了三种等能量和等蛋白的实验日粮作为发酵底物：对照处理（CON组）为不含油籽的基础日粮，另外两种处理分别为基础日粮添加100 g/kg干物质（DM）的生全大豆（S组）和50 g/kg DM的生亚麻籽（F组）。数据显示，S组培养液中乙酸、丁酸和总挥发性脂肪酸（VFA）浓度低于F组（P<0.05）。与其他两组相比，F组丙酸浓度有升高趋势（P = 0.094）。F组的产气量高于对照组（P<0.05）。两种油籽处理组中[此处原文缺失相关指标]丰度均较低（P<0.05），[此处原文缺失相关指标]丰度均较高（P<0.05）。F组中[此处原文缺失相关指标]（P = 0.078）最低。我们的研究结果表明，添加亚麻籽可通过丙酸生成代谢、不饱和脂肪酸（FA）生物氢化以及对[此处原文缺失相关指标]的毒性作用抑制甲烷排放，而对于添加大豆籽，甲烷排放更可能通过[此处原文缺失相关指标]调节的不饱和FA生物氢化作用而降低。
