Effects of High-Forage Diets Containing Raw Flaxseeds or Soybean on In Vitro Ruminal Fermentation, Gas Emission, and Microbial Profile.

Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage diets on in vitro ruminal fermentation, gas composition, and microbial profile. Three isoenergetic and isoproteic experimental diets were designed and used as fermentation substrate: control treatment (CON group) was the basal diet lacking oilseeds, the other two treatments were the basal diet supplemented by 100 g/kg dry matter (DM) raw whole soybean (S group) and 50 g/kg DM raw flaxseed (F group), respectively. Data showed that the acetate, butyrate, and total VFA concentration of culture fluids in the S group were lower ( < 0.05) than in the F group. There was a tendency to a higher level ( = 0.094) of propionate concentration in the F group compared with the other two groups. The gas production in the F group was higher ( < 0.05) than in the control group. There was a lower abundance of ( < 0.05) and a greater abundance of ( < 0.05) in both of the two oilseed treatments. ( = 0.078) in the F group was the lowest. Our results suggested that CH emission could be inhibited with flaxseed supplementation by propionate production metabolism, biohydrogenation of unsaturated fatty acid (FA), and toxicity to , while regarding soybean seed supplementation, the emission of CH was more likely to be reduced through biohydrogenation of unsaturated FA modulated by .