Hattab Jasmine, Marruchella Giuseppe, Pallavicini Alberto, Gionechetti Fabrizia, Mosca Francesco, Trachtman Abigail Rose, Lanci Laura, Gabrielli Luigino, Tiscar Pietro Giorgio
Faculty of Veterinary Medicine, University of Teramo, 64100 Teramo, Italy.
Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
Microorganisms. 2021 Nov 9;9(11):2314. doi: 10.3390/microorganisms9112314.
The investigation of bacterial microbiota represents a developing research field in veterinary medicine intended to look for correlations between animal health and the balance within bacterial populations. The aim of the present work was to define the bacterial microbiota of the oral cavity of healthy sows, which had not been thoroughly described so far. In total, 22 samples of oral fluid were collected and analyzed by 16S-rRNA gene sequencing. CLC Genomics Workbench 20.0 (QIAGEN Digital Insights, Aarhus, Denmark) was then used to examine the results. The predominant orders were Lactobacillales, Clostridiales, and Corynebacteriales. Lactobacillaceae, Corynebacteriaceae, Moraxellaceae, Aerococcaceae, and Staphylococcaceae were the most represented families. As regards the most abundant genera, , , , , , , and can be pointed out as the bacterial core microbiota. Sows were also divided into "gestating" and "lactating" groups, and mild differences were found between pregnant and lactating sows. The data herein described represent an original contribution to the knowledge of the porcine bacterial microbiota. Moreover, the choice of sows as experimental animals was strategic for identifying the adult microbial community. These data provide a basis for further studies on the oral bacterial microbiota of pigs.
细菌微生物群的研究是兽医学中一个不断发展的研究领域，旨在寻找动物健康与细菌种群平衡之间的相关性。本研究的目的是确定健康母猪口腔的细菌微生物群，到目前为止，这方面尚未得到充分描述。总共收集了22份口腔液体样本，并通过16S - rRNA基因测序进行分析。然后使用CLC Genomics Workbench 20.0（QIAGEN Digital Insights，丹麦奥胡斯）来检查结果。优势菌目为乳杆菌目、梭菌目和棒杆菌目。乳杆菌科、棒杆菌科、莫拉克斯氏菌科、气球菌科和葡萄球菌科是占比最高的科。至于最丰富的属， 、 、 、 、 、 、 和 可被指出为细菌核心微生物群。母猪也被分为“妊娠”和“哺乳”组，并且在妊娠母猪和哺乳母猪之间发现了细微差异。本文所述的数据是对猪细菌微生物群知识的原创性贡献。此外，选择母猪作为实验动物对于识别成年微生物群落具有重要意义。这些数据为进一步研究猪口腔细菌微生物群提供了基础。
