Kong In Chul, Ko Kyung-Seok, Lee Sohyeon, Koh Dong-Chan, Burlage Robert
Department of Environmental Engineering, Yeungnam University, Gyungsan 38541, Korea.
Groundwater Department, Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), Daejeon 34132, Korea.
Nanomaterials (Basel). 2021 Oct 24;11(11):2822. doi: 10.3390/nano11112822.
Comparison of the effects of metal oxide nanoparticles (NPs; CuO, NiO, ZnO, TiO, and AlO) on different bioluminescence processes was evaluated using two recombinant ( and ) strains of with same inducer exposure. Different sensitivities and responses were observed according to the type of NPs and recombinant strains. EC values were determined. The negative effects on the bioluminescence activity of the strain was greater than for the strains for all NPs tested. EC values for the strain were 1.7- to 6.2-fold lower (corresponding to high inhibition) than for . ZnO NP caused the greatest inhibition among the tested NPs in both strains, showing approximately 11 times less EC of CuO, which appeared as the least inhibited. Although NPs showed different sensitivities depending on the bioluminescence process, similar orders of EC for both strains were observed as follows: ZnO > NiO, AlO > TiO > CuO. More detailed in-depth systematic approaches, including in the field of molecular mechanisms, is needed to evaluate the accurate effect mechanisms involved in both bioluminescence metabolic processes.
使用两种重组（和）菌株，在相同诱导剂暴露条件下，评估了金属氧化物纳米颗粒（NPs；CuO、NiO、ZnO、TiO和AlO）对不同生物发光过程的影响。根据纳米颗粒的类型和重组菌株观察到了不同的敏感性和反应。测定了EC值。对于所有测试的纳米颗粒，对菌株生物发光活性的负面影响大于对菌株的影响。菌株的EC值比低1.7至6.2倍（对应于高抑制）。在两种菌株中，ZnO NP在所测试的纳米颗粒中引起的抑制作用最大，其EC约为CuO的11倍，CuO表现出的抑制作用最小。尽管纳米颗粒根据生物发光过程表现出不同的敏感性，但两种菌株的EC相似顺序如下：ZnO > NiO，AlO > TiO > CuO。需要更详细深入的系统方法，包括分子机制领域的方法，来评估生物发光代谢过程中涉及的准确作用机制。
