Enhanced Performance of Bioelectrodes Made with Amination-Modified Glucose Oxidase Immobilized on Carboxyl-Functionalized Ordered Mesoporous Carbon.

This research reveals the improved performance of bioelectrodes made with amination-modified glucose oxidase (GOx-NH) and carboxyl-functionalized mesoporous carbon (OMC-COOH). Results showed that when applied with 10 mM EDC amination, the functional groups of NH were successfully added to GOx, according to the analysis of H-NMR, elemental composition, and FTIR spectra. Moreover, after the aminated modification, increased enzyme immobilization (124.01 ± 1.49 mg GOx-NH/g OMC-COOH; 2.77-fold increase) and enzyme activity (1.17-fold increase) were achieved, compared with those of non-modified GOx. Electrochemical analysis showed that aminated modification enhanced the peak current intensity of Nafion/GOx-NH/OMC-COOH (1.32-fold increase), with increases in the charge transfer coefficient (0.54), the apparent electron transfer rate constant (2.54 s), and the surface coverage (2.91 × 10 mol·cm). Results showed that GOx-NH/OMC-COOH exhibited impressive electro-activity and a favorable anodic reaction.