用于葡萄糖传感的金纳米颗粒-聚苯胺复合薄膜

Gold nanoparticle-polyaniline composite films for glucose sensing.

作者信息

Pandey Pratibha, Singh S P, Arya Sunil K, Sharma Anju, Datta Monika, Malhotra Bansi D

机构信息

Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.

出版信息

J Nanosci Nanotechnol. 2008 Jun;8(6):3158-63.

PMID:18681062

Abstract

Gold nanoparticles (AuNPs) have been self-assembled onto electrochemically deposited polyaniline (PANI) films on indium-tin-oxide (ITO) coated glass plates to fabricate glucose biosensor. The covalent immobilization of glucose oxidase (GOx) in the near vicinity of gold nanoparticles has been obtained using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS), chemistry between amino groups of PANI and COOH groups of GOx. These AuNPs-PANI/ ITO and GOx/AuNPs-PANI/ITO composite films have been characterized using Fourier transform infra red (FTIR) and cyclic voltammetry (CV) techniques, respectively. The fast electron transfer from the modified PANI surface to electrode is indicated by the observed increase in amperometric response current of these GOx/AuNPs-PANI/ITO bioelectrodes. These GOx/AuNPs-PANI/ITO bioelectrodes exhibit response time of 10 s, linearity from 50 to 300 mg/dl and show value of apparent Michaelis-Menten constant (Km(app)) of 2.2 mM.

摘要

已将金纳米颗粒（AuNPs）自组装到氧化铟锡（ITO）涂层玻璃板上的电化学沉积聚苯胺（PANI）薄膜上，以制备葡萄糖生物传感器。使用N-乙基-N'-(3-二甲基氨基丙基)碳二亚胺（EDC）/N-羟基琥珀酰亚胺（NHS），通过聚苯胺的氨基与葡萄糖氧化酶（GOx）的羧基之间的化学反应，实现了葡萄糖氧化酶在金纳米颗粒附近的共价固定。分别使用傅里叶变换红外（FTIR）和循环伏安法（CV）技术对这些AuNPs-PANI/ITO和GOx/AuNPs-PANI/ITO复合薄膜进行了表征。这些GOx/AuNPs-PANI/ITO生物电极的安培响应电流增加，表明从修饰的聚苯胺表面到电极的快速电子转移。这些GOx/AuNPs-PANI/ITO生物电极的响应时间为10秒，线性范围为50至300mg/dl，表观米氏常数（Km(app)）值为2.2mM。