Nasori Nasori, Farahdina Ulya, Zulfa Vinda Zakiyatuz, Firdhaus Miftakhul, Aziz Ihwanul, Darsono Darsono, Cao Dawei, Wang Zhijie, Endarko Endarko, Rubiyanto Agus
Laboratory Medical Physics and Biophysics, Department of Physics, Faculty of Sciences and Data Analytic, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia.
Occupational and Safety Department, Nahdlatul Ulama University of Surabaya, Surabaya 60237, Indonesia.
Nanomaterials (Basel). 2021 Nov 18;11(11):3108. doi: 10.3390/nano11113108.
The development of silver (Ag) thin films and the fabrication of Ag nanosquare arrays with the use of an anodic aluminum oxide (AAO) template and leaf extracts were successfully carried out using the DC sputtering and spin coating deposition methods. Ag thin films and Ag nanosquare arrays are developed to monitor cancer prognosis due to the correlation between serum albumin levels and prognostic factors, as well as the binding of serum albumin to the surface of these electrodes. Nanosquare structures were fabricated using AAO templates with varying diameters and a gap distance between adjacent unit cells of 100 nm. The nanosquare array with a diameter of 250 nm and irradiated with electromagnetic waves with a wavelength of around 800 nm possessed the greatest electric field distribution compared to the other variations of diameters and wavelengths. The results of the absorption measurement and simulation showed a greater shift in absorption peak wavelength when carried out using the Ag nanosquare array. The absorption peak wavelengths of the Ag nanosquare array in normal blood and blood with cancer lymphocytes were 700-774 nm and 800-850 nm, respectively. The electrochemical test showed that the sensitivity values of the Ag thin-film electrode deposited using DC sputtering, the Ag thin-film electrode deposited using spin coating, and the Ag nanosquare array in detecting PBS+BSA concentration in the cyclic voltammetry (CV) experiment were 1.308 µA mMcm, 0.022 µA mMcm, and 39.917 µA mMcm, respectively. Meanwhile, the sensitivity values of the Ag thin film and the Ag nanosquare array in detecting the PBS+BSA concentration in the electrochemical impedance spectroscopy (EIS) measurement were 6593.76 Ohm·cm/mM and 69,000 Ohm·cm/mM, respectively. Thus, our analysis of the optical and electrochemical characteristics of Ag thin films and Ag nanosquare arrays showed that both can be used as an alternative biomedical technology to monitor the prognosis of blood cancer based on the concentration of serum albumin in blood.
利用直流溅射和旋涂沉积方法，成功实现了银（Ag）薄膜的制备以及使用阳极氧化铝（AAO）模板和植物叶提取物制备Ag纳米方形阵列。由于血清白蛋白水平与预后因素之间的相关性以及血清白蛋白与这些电极表面的结合，Ag薄膜和Ag纳米方形阵列被开发用于监测癌症预后。使用具有不同直径且相邻单元细胞之间间隙距离为100 nm的AAO模板制备纳米方形结构。与其他直径和波长变化相比，直径为250 nm且用波长约800 nm的电磁波照射的纳米方形阵列具有最大的电场分布。吸收测量和模拟结果表明，使用Ag纳米方形阵列时吸收峰波长有更大的偏移。Ag纳米方形阵列在正常血液和含有癌细胞的血液中的吸收峰波长分别为700 - 774 nm和800 - 850 nm。电化学测试表明，在循环伏安法（CV）实验中，直流溅射沉积的Ag薄膜电极、旋涂沉积的Ag薄膜电极以及Ag纳米方形阵列检测PBS + BSA浓度的灵敏度值分别为1.308 µA mMcm、0.022 µA mMcm和39.917 µA mMcm。同时，在电化学阻抗谱（EIS）测量中，Ag薄膜和Ag纳米方形阵列检测PBS + BSA浓度的灵敏度值分别为6593.76 Ohm·cm/mM和69,000 Ohm·cm/mM。因此，我们对Ag薄膜和Ag纳米方形阵列的光学和电化学特性分析表明，两者都可作为一种替代生物医学技术，基于血液中血清白蛋白的浓度来监测血癌的预后。
