NYU Langone Neurosurgery Associates, New York, NY, USA.
Methods Mol Biol. 2022;2393:225-236. doi: 10.1007/978-1-0716-1803-5_12.
Stimulated Raman histology (SRH) images are created by the label-free, nondestructive imaging of tissue using stimulated Raman scattering (SRS) microscopy. In a matter of seconds, these images provide real-time histologic information on biopsied tissue in the operating room. SRS microscopy uses two lasers (pump beam and Stokes beam) to amplify the Raman signal of specific chemical bonds found in macromolecules (lipids, proteins, and nucleic acids) in these tissues. The concentrations of these macromolecules are used to produce image contrast. These images are acquired and displayed using an imaging system with five main components: (1) fiber coupled microscope, (2) dual-wavelength fiber-laser module, (3) laser control module, (4) microscope control module, and (5) a computer. This manuscript details how to assemble the dual-wavelength fiber-laser module and how to generate an SRH image.
受激拉曼组织学（SRH）图像是通过使用受激拉曼散射（SRS）显微镜对组织进行无标记、非破坏性成像而创建的。在几秒钟内，这些图像可实时提供手术室中活检组织的组织学信息。SRS 显微镜使用两束激光（泵浦光束和斯托克斯光束）来放大这些组织中大分子（脂质、蛋白质和核酸）中特定化学键的拉曼信号。这些大分子的浓度用于产生图像对比度。这些图像是使用具有五个主要组件的成像系统采集和显示的：（1）光纤耦合显微镜，（2）双波长光纤激光模块，（3）激光控制模块，（4）显微镜控制模块和（5）计算机。本文详细介绍了如何组装双波长光纤激光模块以及如何生成 SRH 图像。
