活体容积引导放射治疗的定量生物发光断层扫描。
Quantitative Bioluminescence Tomography for In Vivo Volumetric-Guided Radiotherapy.
机构信息
Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University, Baltimore, MD, USA.
Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
出版信息
Methods Mol Biol. 2022;2393:701-731. doi: 10.1007/978-1-0716-1803-5_38.
Several groups, including ours, have initiated efforts to develop small-animal irradiators that mimic radiation therapy (RT) for human treatment. The major image modality used to guide irradiation is cone-beam computed tomography (CBCT). While CBCT provides excellent guidance capability, it is less adept at localizing soft tissue targets growing in a low image contrast environment. In contrast, bioluminescence imaging (BLI) provides strong image contrast and thus is an attractive solution for soft tissue targeting. However, commonly used 2D BLI on an animal surface is inadequate to guide irradiation, because optical transport from an internal bioluminescent tumor is highly susceptible to the effects of optical path length and tissue absorption and scattering. Recognition of these limitations led us to integrate 3D bioluminescence tomography (BLT) with the small animal radiation research platform (SARRP). In this chapter, we introduce quantitative BLT (QBLT) with the advanced capabilities of quantifying tumor volume for irradiation guidance. The detail of system components, calibration protocol, and step-by-step procedure to conduct the QBLT-guided irradiation are described.
包括我们在内的几个小组已经开始努力开发模拟人类治疗的辐射治疗（RT）的小动物辐照器。用于引导辐照的主要成像方式是锥形束计算机断层扫描（CBCT）。虽然 CBCT 提供了出色的引导能力，但它在定位在低图像对比度环境中生长的软组织靶标方面并不擅长。相比之下，生物发光成像（BLI）提供了很强的图像对比度，因此是软组织靶向的有吸引力的解决方案。然而，在动物表面上常用的二维 BLI 不足以进行辐照引导，因为来自内部生物发光肿瘤的光学传输非常容易受到光程和组织吸收和散射的影响。对这些限制的认识促使我们将三维生物发光断层扫描（BLT）与小动物辐射研究平台（SARRP）集成在一起。在本章中，我们介绍了具有定量肿瘤体积的定量 BLT（QBLT），以用于辐照引导。描述了系统组件的详细信息、校准协议以及进行 QBLT 引导辐照的逐步过程。
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