Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Do Sul, Campo Grande, MS, 79100-510, Brazil.
Instituto de Química, Universidade Federal de Mato Grosso Do Sul, Campo Grande, MS, 79074-460, Brazil.
Mikrochim Acta. 2021 Nov 27;188(12):437. doi: 10.1007/s00604-021-05102-7.
A new contact stamping method for fabrication of paper-based analytical devices (PADs) is reported. It uses an all-purpose acrylic varnish and 3D-printed stamps to pattern hydrophobic structures on paper substrates. The use of 3D printing allows quickly prototyping the desired stamp shape without resorting to third-party services, which are often expensive and time consuming. To the best of our knowledge, this is the first report regarding the use of this material for creation of hydrophobic barriers in paper substrates, as well as this 3D printing-based stamping method. The acrylic varnish was characterized and the features of the stamping method were studied. The PADs developed here presented better compatibility with organic solvents and surfactants compared with similar protocols. Furthermore, the use of this contact stamping method for fabrication of paper electrochemical devices was also possible, as well as multiplexed microfluidic devices for lateral flow testing. The analytical applicability of the varnish-based PADs was demonstrated through the image-based colorimetric quantification of iron in pharmaceutical samples. A limit of detection of 0.61 mg L was achieved. The results were compared with spectrophotometry for validation and presented great concordance (relative error was < 5% and recoveries were between 104 and 108%). Thus, taking into account the performance of the devices explored here, we believe this novel contact stamping method is a very interesting alternative for production of PADs, exhibiting great potentiality. In addition, this work brings a new application of 3D printing in analytical sciences.
一种用于制备纸质分析器件（PADs）的新型接触压印方法被报道。它使用通用的亚克力清漆和 3D 打印的模具在纸质基底上形成疏水结构。3D 打印的使用允许快速原型设计所需的模具形状，而无需求助于通常昂贵且耗时的第三方服务。据我们所知，这是首次报道将这种材料用于在纸质基底上创建疏水屏障，以及这种基于 3D 打印的压印方法。对亚克力清漆进行了表征，并研究了压印方法的特点。与类似的方案相比，这里开发的 PADs 与有机溶剂和表面活性剂的兼容性更好。此外，还可以使用这种接触压印方法来制造纸电化学器件，以及用于侧向流动测试的多路复用微流控器件。通过对药物样品中铁的基于图像的比色定量，证明了基于清漆的 PAD 的分析适用性。实现了 0.61mg L 的检测限。为了验证，将结果与分光光度法进行了比较，结果显示出很好的一致性（相对误差<5%，回收率在 104%至 108%之间）。因此，考虑到所探索的器件的性能，我们认为这种新型接触压印方法是生产 PAD 的一种非常有趣的替代方法，具有很大的潜力。此外，这项工作为分析科学中的 3D 打印带来了新的应用。
