School of Life Sciences, Qinghai Normal University, Xining, 810008, China; Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, 810016, China; Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province, Qinghai Normal University, Xining, 810008, China.
School of Life Sciences, Qinghai Normal University, Xining, 810008, China.
J Plant Physiol. 2022 Jan;268:153575. doi: 10.1016/j.jplph.2021.153575. Epub 2021 Nov 20.
Psammochloa villosa is a desert plant growing in Northwest China with considerable resistance to abiotic stress, including drought, cold, and salt. To facilitate future studies of stress resistance in Psammochloa villosa, we sought to establish a suite of reference (or housekeeping) genes for utilization within future gene expression studies. Specifically, we selected nine candidate genes based on prior studies and new transcriptomic data for P. villosa, and we evaluated their expression stability in three different tissues of P. villosa under different treatments simulating abiotic stress conditions using four different bioinformatics assessments. Our results showed that TIP41 (TIP41-like family protein) was the most stable reference gene in drought- and salt-stressed leaves and salt-stressed stems, ELF-1α (elongation factor 1-α) was the most stable in cold-stressed leaves and drought- and salt-stressed roots, ACT (actin) was the most stable in drought-stressed stems, TUA (α-tubulin) was the most stable in cold-stressed stems, and 18S rRNA (18S ribosomal RNA) was the most stable in cold-stressed roots. Additionally, we tested the utility of these candidate reference genes to detect the expression pattern of P5CS (Δ-pyrroline-5-carboxylate synthetase), which is a drought-related gene. This study is the first report on selecting and validating reference genes of P. villosa under various stress conditions and will benefit future investigations of the genomic mechanisms of stress resistance in this ecologically important species.
沙蓬是一种生长在中国西北地区的荒漠植物，具有相当强的抗逆性，包括干旱、寒冷和盐胁迫。为了方便未来对沙蓬抗逆性的研究，我们试图建立一套参考（或管家）基因，以便在未来的基因表达研究中使用。具体来说，我们根据先前的研究和沙蓬的新转录组数据选择了九个候选基因，并使用四种不同的生物信息学评估方法，在模拟非生物胁迫条件下，评估了它们在沙蓬的三个不同组织中的表达稳定性。我们的结果表明，在干旱和盐胁迫的叶片以及盐胁迫的茎中，TIP41（TIP41 样家族蛋白）是最稳定的参考基因；在冷胁迫的叶片和干旱及盐胁迫的根中，ELF-1α（延伸因子 1-α）是最稳定的；在干旱胁迫的茎中，ACT（肌动蛋白）是最稳定的；在冷胁迫的茎中，TUA（α-微管蛋白）是最稳定的；在冷胁迫的根中，18S rRNA（18S 核糖体 RNA）是最稳定的。此外，我们还测试了这些候选参考基因检测 P5CS（Δ-吡咯啉-5-羧酸合成酶）表达模式的实用性，P5CS 是一个与干旱相关的基因。本研究首次报道了在各种胁迫条件下沙蓬参考基因的选择和验证，将有助于未来对该生态重要物种抗逆性基因组机制的研究。
