Postgraduate Program, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Pharmacy Program, Sekolah Tinggi Ilmu Kesehatan Harapan Ibu, Jambi, Indonesia.
Asian Pac J Cancer Prev. 2021 Nov 1;22(11):3447-3453. doi: 10.31557/APJCP.2021.22.11.3447.
This study aimed to identify the distribution of M2 macrophage and Treg in Nasopharyngeal Carcinoma (NPC) tumor tissue samples. The presence of these two groups of cells was further correlated to clinical stage, tumor size, the lymphatic node involvement, and metastasis.
The total of 50 formalin-fixed paraffin-embedded (FFPE) NPC tissue samples was collected retrospectively (27 samples) and prospectively (23 samples). Samples were FFPE tissue slices. Immunohistochemistry was done on the FFPE tissue slides using anti-CD-163 and anti-FoxP-3 antibodies for M2 macrophage and Treg detection, respectively. The M2 macrophage interpretation was performed by eye-balling method and the score was divided into 0 (negative), 1 (scant), 2 (focal), and 3 (abundant). The average number of Treg FOXP3+ cells in 5 high power fields (HPF) was calculated. The relationship of M2 macrophage and Treg was tested with Spearman's correlation. The relationship between M2 macrophage and Treg with clinical stage, tumor size, node involvement and metastasis was tested by chi square, with p<0.1.
M2 macrophage and Treg were positive correlated (r=0.469, p<0.001). The presence of M2 macrophage and regulatory T cell (Treg) was significantly correlated to tumor size (p= 0.091 for M2 macrophage and p=0.022 for Treg) and clinical stage (p= 0.030 for M2 macrophage and p= 0.002 for Treg), but did not correlate with lymphatic node involvement and metastasis.
In Epstein-Barr virus related NPC tumor microenvironment, the presence of M2 macrophage was correlated with Treg, and both types of the cells were correlated with tumor size and clinical stages.
本研究旨在鉴定鼻咽癌（NPC）肿瘤组织样本中 M2 巨噬细胞和 Treg 的分布。进一步将这两组细胞的存在与临床分期、肿瘤大小、淋巴结受累和转移相关联。
共回顾性收集 50 例福尔马林固定石蜡包埋（FFPE）NPC 组织样本（27 例）和前瞻性收集 23 例 FFPE NPC 组织样本。使用抗 CD-163 和抗 FoxP-3 抗体对 FFPE 组织切片进行免疫组织化学染色，以分别检测 M2 巨噬细胞和 Treg。M2 巨噬细胞的解释通过目测法进行，评分分为 0（阴性）、1（稀疏）、2（局灶）和 3（丰富）。计算 5 个高倍视野（HPF）中 Treg FOXP3+细胞的平均数量。用 Spearman 相关检验检测 M2 巨噬细胞和 Treg 之间的关系。用卡方检验检测 M2 巨噬细胞和 Treg 与临床分期、肿瘤大小、淋巴结受累和转移之间的关系，p<0.1。
M2 巨噬细胞和 Treg 呈正相关（r=0.469，p<0.001）。M2 巨噬细胞和调节性 T 细胞（Treg）的存在与肿瘤大小显著相关（p=0.091 对 M2 巨噬细胞和 p=0.022 对 Treg）和临床分期（p=0.030 对 M2 巨噬细胞和 p=0.002 对 Treg），但与淋巴结受累和转移无关。
在 Epstein-Barr 病毒相关的 NPC 肿瘤微环境中，M2 巨噬细胞的存在与 Treg 相关，这两种细胞都与肿瘤大小和临床分期相关。
