DMH 诱导的炎症相关结直肠癌大鼠结肠中分布显著的 M2 巨噬细胞。
M2 Macrophage Prominently Distributed in the Rat's Colon of DMH-Induced Inflammation Associated Colorectal Cancer.
机构信息
Faculty of Biology Universitas Gadjah Mada, Yogyakarta, Indonesia.
Digestive Surgery Department of Medical Faculty Universitas Diponegoro, Semarang, Indonesia.
出版信息
Asian Pac J Cancer Prev. 2024 Apr 1;25(4):1357-1362. doi: 10.31557/APJCP.2024.25.4.1357.
OBJECTIVE
The aim of this study is to examine the M1 and M2 macrophages distribution in the rat's colon of DMH-induced inflammation associated colorectal cancer.
METHODS
Colon tissue of three groups of 4 rats that induced using 1,2 dimethylhydrazine (DMH) at 30 mg/kg bw every week for 9, 11, and 13 weeks were used. The M1 and M2 distribution was examined by using antibody anti iNOS for M1 and anti-CD163 for M2 with immunohistochemistry method. The data was presents in figure and table in the form of percentage.
RESULT
M1 macrophage was found in all groups in the low distribution level (25% - 50%), while M2 macrophage was observed in all groups with 100% distribution. In the longer period of DMH induction, M2 macrophages was distributed more abundant.
CONCLUSION
All of the rat's colon showing chronic inflammation that led to the tumorigenesis.
目的
本研究旨在观察 DMH 诱导的炎症相关结直肠癌大鼠结肠中 M1 和 M2 巨噬细胞的分布。
方法
使用每周 30mg/kg bw 的 1,2 二甲基肼（DMH）诱导三组 4 只大鼠的结肠组织，共 9、11 和 13 周。使用免疫组织化学方法用抗 iNOS（M1）和抗 CD163（M2）抗体检测 M1 和 M2 的分布。数据以百分比的形式以图表和表格的形式呈现。
结果
所有组中均观察到 M1 巨噬细胞呈低分布水平（25%-50%），而 M2 巨噬细胞在所有组中均呈 100%分布。在 DMH 诱导时间较长的情况下，M2 巨噬细胞分布更为丰富。
结论
所有大鼠的结肠均表现出慢性炎症，导致肿瘤发生。
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