Institute of Functional Anatomy, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt Universität zu Berlin, Philippstr. 11, 10115, Berlin, Germany.
German Center for Lung Research (DZL), Berlin, Germany.
Respir Res. 2021 Nov 27;22(1):305. doi: 10.1186/s12931-021-01899-2.
The intention of this short primer is to raise your appetite for proper quantitative assessment of lung micro-structure. The method of choice for obtaining such data is stereology. Rooted in stochastic geometry, stereology provides simple and efficient tools to obtain quantitative three-dimensional information based on measurements on nearly two-dimensional microscopic sections. In this primer, the basic concepts of stereology and its application to the lung are introduced step by step along the workflow of a stereological study. The integration of stereology in your laboratory work will help to improve its quality. In a broader context, stereology may also be seen as a contribution to good scientific practice.
本文旨在激发您对肺部微观结构进行适当定量评估的兴趣。获取此类数据的首选方法是体视学。体视学基于随机几何,为基于近二维显微镜切片的测量获取定量三维信息提供了简单而有效的工具。在本入门指南中,将逐步介绍体视学的基本概念及其在肺部中的应用,沿着体视学研究的工作流程进行介绍。将体视学集成到您的实验室工作中有助于提高其质量。从更广泛的角度来看,体视学也可以被视为对良好科学实践的贡献。