Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Athens, Greece.
J Liposome Res. 2022 Mar;32(1):83-91. doi: 10.1080/08982104.2021.1978486. Epub 2021 Nov 29.
The aim of the present study is the development and evaluation of the physicochemical properties of chimeric hydrogenated soya phosphatidylcholine (HSPC) and egg phosphatidylcholine (EggPC) liposomes with incorporated triblock copolymer Poloxamer P407 (P407). The physicochemical assay was held in water HPLC-grade and Foetal Bovine Serum (FBS), in order to determine whether these systems can be used as drug or antigen delivery nanosystems. Dynamic and electrophoretic light scattering (DLS/ELS) techniques were used for the measurement of the hydrodynamic diameter, the polydispersity index, and the ζ-potential of the prepared nanosystems. The incorporation of the P407 resulted in a size reduction of all systems. A decrease in the hydrodynamic diameter and polydispersity index were also found as a result of increasing the storage temperature from 4 °C to 25 °C, attributed to P407. The experiments that were carried out in FBS, showed that the addition of P407 improved systems stealth properties. Concluding, we propose P407 as a promising alternative to PEG in the development of lipid nanoparticles with optimized bio- and shelf-stability.
本研究的目的是开发和评估嵌合氢化大豆磷脂酰胆碱 (HSPC) 和卵磷 脂 (EggPC) 脂质体与掺入的三嵌段共聚物泊洛沙姆 P407 (P407) 的物理化学性质。在水 HPLC 级和胎牛血清 (FBS) 中进行物理化学测定，以确定这些系统是否可用作药物或抗原递药纳米系统。动态和电泳光散射 (DLS/ELS) 技术用于测量制备的纳米系统的水动力直径、多分散指数和 ζ-电位。P407 的掺入导致所有系统的粒径减小。还发现随着储存温度从 4°C 升高到 25°C，水动力直径和多分散指数减小，这归因于 P407。在 FBS 中进行的实验表明，添加 P407 提高了系统的隐身性能。总之，我们提出 P407 是在开发具有优化的生物和货架稳定性的脂质纳米粒时替代 PEG 的有前途的选择。
