一组血小板相关循环长链非编码RNA作为结直肠癌的潜在生物标志物。

A panel of platelet-associated circulating long non-coding RNAs as potential biomarkers for colorectal cancer.

作者信息

Ye Bingqi, Li Fengying, Chen Mengsha, Weng Yu, Qi Chao, Xie Yulin, Zhang Qikun, Ding Huisi, Zhang Jun, Gao Xiangwei

机构信息

Sir Run-Run Shaw Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou 310058, China.

Sir Run-Run Shaw Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou 310058, China; Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 East Qingchun Road, Hangzhou, 310016 Zhejiang, People's Republic of China; Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, 3 East Qingchun Road, Hangzhou, 310016 Zhejiang, People's Republic of China.

出版信息

Genomics. 2022 Jan;114(1):31-37. doi: 10.1016/j.ygeno.2021.11.026. Epub 2021 Nov 27.

DOI:10.1016/j.ygeno.2021.11.026

PMID:34843904

Abstract

Evidence has suggested the potential of tumor-educated platelets as a biomarker trove for cancer diagnostics, but the difficulty in isolation limits its application. Since most of the circulating RNAs are derived from platelets, the change of RNA profile in platelets may lead to altered RNA expression in serum. Here, we identified a panel of platelet-associated long non-coding RNAs (lncRNAs) and evaluated its diagnostic capacity in serum of colorectal cancer (CRC) patients. Four lncRNAs, LNCAROD, SNHG20, LINC00534, and TSPOAP-AS1, were upregulated in both platelets and serum of CRC patients. A binary logistic model derived from them has validated area under roc curve of 0.78 indicating great performance. Furthermore, the expression levels of LNCAROD and TSPOAP-AS1 were correlated with cancer staging and tumor location. Together, our results add novel lncRNA biomarkers to the list of blood tests for CRC diagnostics and provide molecular evidence for the cross-talk between CRC platelets and serum.

摘要

有证据表明，肿瘤驯化血小板作为癌症诊断生物标志物宝库具有潜力，但分离困难限制了其应用。由于大多数循环RNA来源于血小板，血小板中RNA谱的变化可能导致血清中RNA表达改变。在此，我们鉴定了一组血小板相关长链非编码RNA（lncRNA），并评估了其在结直肠癌（CRC）患者血清中的诊断能力。在CRC患者的血小板和血清中，四种lncRNA，即LNCAROD、SNHG20、LINC00534和TSPOAP-AS1均上调。由它们推导的二元逻辑模型已验证roc曲线下面积为0.78，表明性能良好。此外，LNCAROD和TSPOAP-AS1的表达水平与癌症分期和肿瘤位置相关。总之，我们的结果为CRC诊断的血液检测增加了新的lncRNA生物标志物，并为CRC血小板与血清之间的相互作用提供了分子证据。