Laboratory Medicine Center/Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, China.
Laboratory Medicine Center, Nantong Tumor Hospital, 48 Qingnian Road, Nantong 226001, China.
Biosci Rep. 2020 Mar 27;40(3). doi: 10.1042/BSR20191481.
Studies have shown that long non-coding RNAs (lncRNAs) play vital roles in the development of cancer, including colorectal cancer (CRC). Our purpose is to validate the diagnostic value of serum differentiation antagonizing non-protein coding RNA (DANCR) in CRC by focusing on its expression and clinical application. lncRNA expression profiles of CRC patients were obtained and analyzed by repurposing the publically available microarray data. Tissue or serum specimens were obtained from 40 patients with primary CRC, 10 patients with recurrent CRC, 40 patients with colorectal polyps, and 40 healthy controls. It was found that DANCR level in the CRC tissue and serum was significantly increased, and serum DANCR expression was decreased in post-operative patients as compared with that in pre-treatment patients and recurrent patients. In addition, serum DANCR expression was significantly correlated with different TNM stages. Correlation analysis of DANCR and other diagnostic indicators showed that the serum DANCR expression level was significantly correlated with CA199 but not with CEA in CRC patients. As for diagnostic efficiency by ROC analysis, the area under the curve (AUC) of serum DANCR was higher than that of CEA and CA199 in CRC group vs. colorectal polyp group. Simultaneous detection of DANCR, CEA and CA199 yielded the highest sensitivity and AUC as compared with either of them alone. Taken together, serum DANCR was up-regulated in CRC patients and high expression of DANCR may prove to be a potential biomarker for the diagnosis of CRC.
研究表明，长链非编码 RNA（lncRNA）在癌症的发展中发挥着重要作用，包括结直肠癌（CRC）。我们的目的是通过关注其表达和临床应用，验证血清分化拮抗非蛋白编码 RNA（DANCR）在 CRC 中的诊断价值。通过重新利用公开的微阵列数据获得了 CRC 患者的 lncRNA 表达谱，并进行了分析。从 40 例原发性 CRC 患者、10 例复发性 CRC 患者、40 例结肠息肉患者和 40 例健康对照者中获得组织或血清标本。结果发现，CRC 组织和血清中的 DANCR 水平显著升高，与术前患者和复发性患者相比，术后患者血清 DANCR 表达降低。此外，血清 DANCR 表达与不同的 TNM 分期显著相关。DANCR 与其他诊断指标的相关性分析表明，CRC 患者血清 DANCR 表达水平与 CA199 显著相关，但与 CEA 不相关。ROC 分析的诊断效率显示，CRC 组与结肠息肉组相比，血清 DANCR 的 AUC 高于 CEA 和 CA199。同时检测 DANCR、CEA 和 CA199 的敏感性和 AUC 均高于单独检测任何一种标志物。综上所述，CRC 患者血清 DANCR 呈上调表达，DANCR 的高表达可能证明是 CRC 诊断的潜在生物标志物。
