Department of Gastrointestinal Surgery, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443000, Hubei, China.
Department of Hematology, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443000, Hubei, China.
Cancer Biomark. 2017 Dec 6;20(4):417-424. doi: 10.3233/CBM-170179.
To evaluate the diagnostic efficacy and prognostic value of serum long non-coding RNA (lncRNA) HIF 1alpha-antisense RNA 1 (HIF1A-AS1) in patients with colorectal carcinoma (CRC). We obtained serum samples from 151 CRC patients and 160 health controls. Serum level of HIF1A-AS1 was detected via real-time PCR (RT-PCR). Receiver operating characteristics (ROC) curve analysis was conducted to determine the diagnostic value of HIF1A-AS1. Then HIF1A-AS1 in CRC was divided into high- and low-expression groups, and the associations of the HIF1A-AS1 serum level with clinicopathological features and prognosis were analyzed. Serum level of HIF1A-AS1 was significantly increased from CRC patients as compared to those of health controls (P< 0.05). ROC curve analysis revealed a relative high diagnostic performance of HIF1A-AS1 to distinguish CRC from health controls, with the area under the curves (AUC) of 0.960 (95% CI: 0.940 ∼ 0.980; P< 0.001). Kaplan-Meier analysis showed that differentiation degree, tumor size, TNM stage, T stage, N stage, M stage and serum level of HIF1A-AS1 were all linked to CRC prognosis (All P< 0.05). Compared to CRC patients with low HIF1A-AS1 expression, high expression of patients were associated with a shorter 5-year-survival rate (P< 0.001). Multivariate Cox regression analysis revealed that lower differentiation degree, tumor > 5 cm and higher expression of HIF1A-AS1 were independent risk factors affecting the survival rate of patients with CRC (P< 0.05). Our results illustrated that elevated serum HIF1AAS1 could be clinically functioned as a potential biomarker for CRC diagnoses and prognosis.
为评估血清长链非编码 RNA（lncRNA）HIF1alpha-antisense RNA 1（HIF1A-AS1）在结直肠癌（CRC）患者中的诊断效能和预后价值。我们收集了 151 例 CRC 患者和 160 例健康对照者的血清样本。采用实时荧光定量 PCR（RT-PCR）检测血清 HIF1A-AS1 水平。绘制受试者工作特征（ROC）曲线分析 HIF1A-AS1 的诊断价值。然后将 CRC 中的 HIF1A-AS1 分为高表达组和低表达组，分析 HIF1A-AS1 血清水平与临床病理特征及预后的关系。与健康对照组相比，CRC 患者血清 HIF1A-AS1 水平显著升高（P<0.05）。ROC 曲线分析显示，HIF1A-AS1 对区分 CRC 与健康对照组具有较高的诊断性能，曲线下面积（AUC）为 0.960（95%CI：0.940~0.980；P<0.001）。Kaplan-Meier 分析表明，分化程度、肿瘤大小、TNM 分期、T 分期、N 分期、M 分期和 HIF1A-AS1 血清水平均与 CRC 预后相关（均 P<0.05）。与 HIF1A-AS1 低表达的 CRC 患者相比，高表达患者的 5 年生存率较短（P<0.001）。多因素 Cox 回归分析显示，低分化程度、肿瘤>5cm 和 HIF1A-AS1 高表达是影响 CRC 患者生存率的独立危险因素（P<0.05）。本研究表明，血清 HIF1A-AS1 水平升高可能具有作为 CRC 诊断和预后的潜在临床标志物的作用。
