Hsiao Ta-Chih, Cheng Po-Ching, Chi Kai Hsien, Wang Hung-Yang, Pan Shih-Yu, Kao Ching, Lee Yueh-Lun, Kuo Han-Pin, Chung Kian Fan, Chuang Hsiao-Chi
Graduate Institute of Environmental Engineering, National Taiwan University, Taipei, Taiwan.
Department of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Center for International Tropical Medicine, School of Medicine, Taipei Medical University, Taipei, Taiwan.
J Hazard Mater. 2022 Feb 5;423(Pt B):127243. doi: 10.1016/j.jhazmat.2021.127243. Epub 2021 Sep 24.
The significance of this work is that ambient PM is a direct transmission mode for influenza virus infection to the human alveolar epithelium. The concentration of PM was 11.7 ± 5.5 μg/m in Taipei during 24 December 2019-13 January 2020. Approximately 79% of inhaled PM is able to reach the upper-to-lower airway, and 47% of PM is able to reach the alveolar epithelium for influenza virus infection. Influenza A and B viruses were detected in PM on 9 days, and the influenza A/H5 virus was detected on 15 days during the study period. FL and Pyr were negatively correlated with the influenza A virus. D(ah)P and Acp were positively correlated with the influenza B and A/H5 viruses, respectively. Cd, V, and Zn were positively correlated with the influenza A, B, and A/H5 viruses, respectively. Next, influenza A, B, and A/H5 viral plasmids interacted with carbon black, HO, DEPs, and UD. We observed that HO significantly decreased levels of complementary DNA of the three influenza viruses. DEPs and UD significantly decreased influenza A and A/H5 viral levels. In conclusion, chemicals in PM may play vital roles in terms of viable influenza virus in the atmosphere.
这项工作的意义在于，环境颗粒物是流感病毒感染人类肺泡上皮的一种直接传播方式。2019年12月24日至2020年1月13日期间，台北的颗粒物浓度为11.7±5.5μg/m。吸入的颗粒物中约79%能够到达上、下呼吸道，47%的颗粒物能够到达肺泡上皮引发流感病毒感染。在研究期间，有9天在颗粒物中检测到甲型和乙型流感病毒，15天检测到甲型H5流感病毒。氟利昂和芘与甲型流感病毒呈负相关。二噁英和邻苯二甲酸二辛酯分别与乙型流感病毒和甲型H5流感病毒呈正相关。镉、钒和锌分别与甲型、乙型和甲型H5流感病毒呈正相关。接下来，甲型、乙型和甲型H5流感病毒质粒与炭黑、羟基自由基、柴油机排气微粒和城市灰尘发生相互作用。我们观察到，羟基自由基显著降低了三种流感病毒的互补DNA水平。柴油机排气微粒和城市灰尘显著降低了甲型和甲型H5流感病毒水平。总之，颗粒物中的化学物质可能在大气中存活的流感病毒方面发挥重要作用。
