State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, 450001 Zhengzhou, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China.
J Hazard Mater. 2022 Feb 5;423(Pt B):127258. doi: 10.1016/j.jhazmat.2021.127258. Epub 2021 Sep 20.
Research on microplastic pollution of terrestrial soils is catching up with the aquatic environment, especially agricultural soil systems. Plastic residues have caused various environmental problems in mulch film extensively used agricultural areas. However, studies focusing specifically on the potential influence of mulch film residues on the metabolic cycle of soil systems have yet to be conducted. Here, high-throughput sequencing combined with metabolomics were first used to study the effects of residual mulch on soil microbial communities and related metabolic functions. Plastic film treatment did not significantly affect soil physicochemical properties including pH, organic matter and nitrogen, etc in short term. However, it did significantly changed overall community structure of soil bacteria, and interfered with complexity of soil bacterial symbiosis networks; exposure time and concentration of residues were particularly important factors affecting community structure. Furthermore, metabolomics analysis showed that film residue significantly changed soil metabolite spectrum, and interfered with basic carbon and lipid metabolism, and also affected basic cellular processes such as membrane transport and, in particular, interfered with the biosynthesis of secondary metabolites, as well as, biodegradation and metabolism of xenobiotics. Additionally, through linear discriminant and collinear analysis, some new potential microplastic degrading bacteria including Nitrospira, Nocardioidaceae and Pseudonocardiaceae have been excavated.
土壤中微塑料污染的研究正在迎头赶上水生环境，特别是农业土壤系统。在广泛使用覆盖膜的农业地区，塑料残留物已经造成了各种环境问题。然而，专门针对覆盖膜残留物对土壤系统代谢循环潜在影响的研究尚未开展。在这里，我们首次使用高通量测序结合代谢组学研究了残留覆盖膜对土壤微生物群落和相关代谢功能的影响。短期内，塑料薄膜处理并没有显著影响土壤的理化性质，包括 pH 值、有机质和氮等。然而，它确实显著改变了土壤细菌的整体群落结构，并干扰了土壤细菌共生网络的复杂性；暴露时间和残留物浓度是影响群落结构的特别重要因素。此外，代谢组学分析表明，薄膜残留物显著改变了土壤代谢物谱，干扰了基本的碳和脂质代谢，还影响了基本的细胞过程，如膜运输，特别是干扰了次生代谢物的生物合成以及异生物质的生物降解和代谢。此外，通过线性判别和共线性分析，挖掘到了一些新的潜在的能够降解微塑料的细菌，包括硝化螺旋菌科、诺卡氏菌科和拟诺卡氏菌科。
