State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, 450001 Zhengzhou, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China.
Ecotoxicol Environ Saf. 2022 Mar 15;233:113338. doi: 10.1016/j.ecoenv.2022.113338. Epub 2022 Feb 26.
As microplastics became the focus of global attention, the hazards of plastic plasticizers (PAEs) have gradually attracted people's attention. Agricultural soil is one of its hardest hit areas. However, current research of its impact on soil ecology only stops at the microorganism itself, and there is still lack of conclusion on the impact of soil metabolism. To this end, three most common PAEs (Dimethyl phthalate: DMP, Dibutyl phthalate: DBP and Bis (2-ethylhexyl) phthalate: DEHP) were selected and based on high-throughput sequencing and metabolomics platforms, the influence of PAEs residues on soil metabolic functions were revealed for the first time. PAEs did not significantly changed the alpha diversity of soil bacteria in the short term, but changed their community structure and interfered with the complexity of community symbiosis network. Metabolomics indicated that exposure to DBP can significantly change the soil metabolite profile. A total of 172 differential metabolites were found, of which 100 were up-regulated and 72 were down-regulated. DBP treatment interfered with 43 metabolic pathways including basic metabolic processes. In particular, it interfered with the metabolism of residual steroids and promoted the metabolism of various plasticizers. In addition, through differential labeling and collinear analysis, some bacteria with the degradation potential of PAEs, such as Gordonia, were excavated.
随着微塑料成为全球关注的焦点，塑料增塑剂（PAEs）的危害逐渐引起人们的关注。农业土壤是其受影响最严重的地区之一。然而，目前对其对土壤生态影响的研究仅停留在微生物本身，对土壤代谢的影响仍缺乏结论。为此，选择了三种最常见的 PAEs（邻苯二甲酸二甲酯：DMP、邻苯二甲酸二丁酯：DBP 和邻苯二甲酸二（2-乙基己基）酯：DEHP），并基于高通量测序和代谢组学平台，首次揭示了 PAEs 残留对土壤代谢功能的影响。PAEs 在短期内并没有显著改变土壤细菌的 alpha 多样性，但改变了它们的群落结构并干扰了群落共生网络的复杂性。代谢组学表明，DBP 的暴露可以显著改变土壤代谢物图谱。共发现 172 个差异代谢物，其中 100 个上调，72 个下调。DBP 处理干扰了包括基本代谢过程在内的 43 个代谢途径。特别是，它干扰了甾体残留的代谢，促进了各种增塑剂的代谢。此外，通过差异标记和共线性分析，挖掘到了一些具有 PAEs 降解潜力的细菌，如 Gordonia。
