Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, HP, India; Academy of Scientific and Innovative Research, Ghaziabad 201002, Uttar Pradesh, India.
Dietetics& Nutrition Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, HP, India; Academy of Scientific and Innovative Research, Ghaziabad 201002, Uttar Pradesh, India.
Food Chem. 2022 Mar 30;373(Pt B):131561. doi: 10.1016/j.foodchem.2021.131561. Epub 2021 Nov 9.
Camellia sinensis (tea) is an evergreen plant having bioactive compounds associated with various pharmacological effects, including anti-cancerous activity. These phytochemicals are variedly distributed in plant tissues. A detailed study to understand chemical composition within the economically underutilized tea tissues is required to generate value. Therefore, a comprehensive chemical profiling of underutilized C. sinensis parts [coarse leaves, flowers, fruits (immature);n = 9] was performed by NMR techniques. NMR (1D and 2D) spectroscopy ambiguously identified and quantified fifty-seven metabolites (Coarse leaves: 35, flowers; 42, immature fruits; 45). The statistical analysis showed apparent tissue-specific similarities (26 metabolites) and variations. Further, HPLC-DAD revealed absolute quantification of catechins, caffeine and theanine among the different parts of C. sinensis. Moreover, cytotoxicity studies of tea tissues against colorectal cancer cell lines showed anticancer potentials. This chemical information and anticancer activity of underutilized C. sinensis parts will help to develop value added nutraceutical and cosmeceutical products.
茶树（茶）是一种常绿植物，含有与各种药理作用相关的生物活性化合物，包括抗癌活性。这些植物化学物质在植物组织中分布不均。为了创造价值，需要对经济上未充分利用的茶组织进行详细研究，以了解其化学成分。因此，采用 NMR 技术对未充分利用的茶树部分[粗叶、花、未成熟果实（n=9）]进行了全面的化学成分分析。NMR（1D 和 2D）光谱技术模糊地鉴定和定量了 57 种代谢物（粗叶：35 种，花：42 种，未成熟果实：45 种）。统计分析显示，不同组织之间存在明显的组织特异性相似性（26 种代谢物）和差异。此外，HPLC-DAD 还揭示了不同茶树部分儿茶素、咖啡因和茶氨酸的绝对定量。此外，茶组织对结直肠癌细胞系的细胞毒性研究显示出抗癌潜力。这些未充分利用的茶树部分的化学信息和抗癌活性将有助于开发有附加值的营养保健品和化妆品。
