Yu C L, Haskard D, Cavender D, Ziff M
J Immunol. 1986 Jan;136(2):569-73.
Preincubation of human umbilical vein endothelial cell (EC) monolayers with 1 ng to 10 micrograms/ml lipopolysaccharide (LPS) increased the binding of T lymphocytes to EC. The effect was maximal at LPS concentrations of 0.1 to 10 micrograms/ml, and occurred with LPS derived from Escherichia coli (serotypes 0111:B4 and 0127:B8), Shigella flexneri (serotype 2a), Serratia marcescens (serotype 0:3), and Yersinia entercolitica (serotype 0:3). The increased binding appeared to be mediated primarily through an action on EC; preincubation of T cells rather than EC with LPS did not lead to enhanced binding. The onset of enhanced binding was very rapid, being observed after 2 to 3 min of preincubation and becoming maximal after 1 hr. EC were unresponsive to LPS after fixation with 2% paraformaldehyde-L-lysine-periodate and also when the LPS was incubated with EC at 4 degrees C. Enhanced binding was seen with lipid A and with LPS from Salmonella minnesota Re 595 (mainly lipid A) and was abolished by conjugation with polymyxin B. The observed increase in the binding of lymphocytes to EC exposed to LPS suggests that the lymphocytopenia induced by endotoxemia may result from augmentation of the adherence of lymphocytes to altered endothelium.
将人脐静脉内皮细胞（EC）单层与1纳克至10微克/毫升的脂多糖（LPS）预孵育，可增加T淋巴细胞与EC的结合。在LPS浓度为0.1至10微克/毫升时，这种效应最为明显，且来自大肠杆菌（血清型0111:B4和0127:B8）、福氏志贺菌（血清型2a）、粘质沙雷氏菌（血清型0:3）和小肠结肠炎耶尔森菌（血清型0:3）的LPS均可产生这种效应。结合增加似乎主要是通过对EC的作用介导的；用LPS预孵育T细胞而非EC不会导致结合增强。结合增强的起始非常迅速，预孵育2至3分钟后即可观察到，1小时后达到最大值。用2%多聚甲醛-L-赖氨酸-高碘酸盐固定EC后，EC对LPS无反应，当LPS在4℃下与EC孵育时也无反应。脂质A以及来自明尼苏达沙门氏菌Re 595的LPS（主要是脂质A）可观察到结合增强，且与多粘菌素B结合后这种增强被消除。观察到淋巴细胞与暴露于LPS的EC结合增加，这表明内毒素血症诱导的淋巴细胞减少可能是由于淋巴细胞与改变的内皮细胞粘附增加所致。
