木本寄主中柑橘类病毒的研究与检测。

Study and Detection of Citrus Viroids in Woody Hosts.

机构信息

United States Department of Agriculture, Agricultural Research Service (USDA-ARS), National Clonal Germplasm Repository for Citrus and Dates, Riverside, CA, USA.

Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.

出版信息

Methods Mol Biol. 2022;2316:3-21. doi: 10.1007/978-1-0716-1464-8_1.

DOI:10.1007/978-1-0716-1464-8_1

PMID:34845680

Abstract

Biological indexing is based upon the ability of certain plants, referred to as indicator plants or indicators, to produce specific symptoms when inoculated with a pathogen using mechanical means or grafting. In the case of citrus viroids, clonal indicators are grafted on to vigorous rootstocks such as rough lemon (Citrus × granulata Raf.). The 'Arizona-861-S-1' citron clonal indicator (C. medica L.) can detect and bioamplify all citrus viroids; however, for specific citrus variants of the hop stunt viroid (i.e., CVd-IIb and CVd-IIc), the clonal indicator 'Parson's special # 9' mandarin (C. reticulata Blanco) is preferred. Inoculation techniques and symptom expression are described in detail. Other supporting elements, such as greenhouse conditions and propagation techniques, are also presented.

摘要

生物指标是基于某些植物的能力，这些植物被称为指示植物或指示剂，当使用机械手段或嫁接将病原体接种到植物上时，它们能够产生特定的症状。在柑橘类病毒的情况下，无性系指示植物被嫁接到粗柠檬（Citrus × granulata Raf.）等旺盛的砧木上。‘Arizona-861-S-1’香橼无性系指示植物（C. medica L.）可以检测和生物扩增所有柑橘类病毒；然而，对于特定的柑橘类Hop 矮化病毒变体（即 CVd-IIb 和 CVd-IIc），则更喜欢无性系指示植物‘Parson's special # 9’橘（C. reticulata Blanco）。本文详细描述了接种技术和症状表现。还介绍了其他支持元素，如温室条件和繁殖技术。