基于高通量测序数据的柑橘类病毒属病毒的计算检测
An In Silico Detection of a Citrus Viroid from Raw High-Throughput Sequencing Data.
机构信息
Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
Institute of Biosecurity and Microbial Forensics, Oklahoma State University, Stillwater, OK, USA.
出版信息
Methods Mol Biol. 2022;2316:275-283. doi: 10.1007/978-1-0716-1464-8_23.
E-probe Diagnostic for Nucleic Acid Analysis (EDNA) is a user-friendly bioinformatic tool that has been adapted for the detection and identification of citrus exocortis viroid (CEVd). Here, we describe the procedures for RNA extraction from citrus tissues, library and sequencing preparation, and the utilization of EDNA Mi-Finder online platform on raw high-throughput sequencing (HTS) data.
E-probe 核酸分析诊断（EDNA）是一个用户友好的生物信息学工具，已经被改编用于检测和鉴定柑橘外皮层坏死病毒（CEVd）。在这里，我们描述了从柑橘组织中提取 RNA、文库和测序准备的程序，以及在原始高通量测序（HTS）数据上使用 EDNA Mi-Finder 在线平台的过程。
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