巴勒斯坦地区柑橘外果皮脆裂病毒（CEVd）、柑橘裂皮类病毒-III（CVd-III）和柑橘裂皮类病毒-IV（CVd-IV）的分子检测。

Molecular detection of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in Palestine.

机构信息

Biology and Biotechnology Department, An-Najah National University, Nablus, Palestine.

National Agricultural Research Center (NARC), Jenin, Palestine.

出版信息

Sci Rep. 2024 Jan 3;14(1):423. doi: 10.1038/s41598-023-50271-5.

DOI:10.1038/s41598-023-50271-5

PMID:38172610

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10764322/

Abstract

Citrus hosts various phytopathogens that have impacted productivity, including viroids. Missing data on the status of viroids in citrus in Palestine were not reported. This study was aimed to detect any of Citrus exocortis viroid (CEVd), Citrus viroid-III (CVd-III), and Citrus viroid-IV (CVd-IV) in the Palestinian National Agricultural Research Center (NARC) germplasm collection Field inspections found symptoms such as leaf epinasty; vein discoloration, and bark cracking on various citrus varieties. RT-PCR revealed a significant prevalence of CVd-IV; CEVd and CVd-III (47%, 31%, and 22%; respectively). CVd-III variants with 91.3% nucleic acid sequence homology have been reported. The sequence of each viroid were deposited in GenBank as (OP925746 for CEVd, OP902248 and OP902249 for CVd-III-PS-1 and -PS-2 isolates, and OP902247 for CVd-IV). This was the first to report three of citrus viroids in Palestine, appealing to apply of phytosanitary measures to disseminate healthy propagating materials free from viroids.

摘要

柑橘类植物易受多种植物病原体的影响，从而降低其产量，类病毒就是其中之一。此前并未报告过巴勒斯坦柑橘类植物中类病毒的相关数据。本研究旨在检测巴勒斯坦国家农业研究中心（NARC）种质资源圃中是否存在柑橘外果皮脆裂病毒（CEVd）、柑橘脉斑驳病毒-III（CVd-III）和柑橘脉斑驳病毒-IV（CVd-IV）。田间调查发现，多种柑橘品种出现叶片内卷、叶脉变色和树皮开裂等症状。实时 RT-PCR 检测结果显示，CVd-IV、CEVd 和 CVd-III 的检出率分别为 47%、31%和 22%。已报道 CVd-III 有 91.3%的核苷酸序列同源性。每条类病毒的序列均已在 GenBank 中注册，CEVd 的注册号为 OP925746，CVd-III-PS-1 和 -PS-2 分离株的注册号为 OP902248 和 OP902249，CVd-IV 的注册号为 OP902247。这是首次在巴勒斯坦报道三种柑橘类病毒，呼吁采取植物检疫措施，传播无病毒的健康繁殖材料。