Koroglu Aydın Pınar, Karabulut-Bulan Omur, Bugan Ilknur, Turkyilmaz Ismet Burcu, Altun Seyhan, Yanardag Refiye
Department of Histology and Embryology, Faculty of Medicine, Halic University, Istanbul, Turkey.
Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey.
Cell Biochem Funct. 2022 Jan;40(1):60-70. doi: 10.1002/cbf.3674. Epub 2021 Nov 29.
Individuals with diabetes have an increased risk of breast, colorectal, pancreatic and prostate cancer. Metformin, an oral biguanide used to treat diabetes, has anti-hyperglycaemic, anti-hyperinsulinemic and antioxidant activities. The effects of metformin on testicular tissue damage in cancer and diabetic + cancer rat models were evaluated histologically, immunohistochemically and biochemically. The diabetic model was produced in Copenhagen rats using a single dose of streptozotocin (65 mg/kg), while prostate cancer was induced through subcutaneous inoculation of 2 × 10 Mat-LyLu cells into the animals. At the end of the experimental period, testicular tissues with a close functional relationship to the prostate were collected. Histological evaluation found moderate to severe damage to testes following the diabetes and cancer process. Histopathological and biochemical impairments were observed in the early stage of prostate cancer, which were increased in the diabetic animals. Metformin administration reversed these injuries and provided substantial protection of the testes. In particular, metformin had protective effects on tissue damage, apoptosis, oxidative stress and antioxidant capacity. This suggests that metformin should be further investigated as a targeted protective drug against prostate cancer-related damage to the testes.
糖尿病患者患乳腺癌、结直肠癌、胰腺癌和前列腺癌的风险增加。二甲双胍是一种用于治疗糖尿病的口服双胍类药物，具有抗高血糖、抗高胰岛素血症和抗氧化活性。通过组织学、免疫组织化学和生物化学方法评估了二甲双胍对癌症和糖尿病合并癌症大鼠模型睾丸组织损伤的影响。使用单剂量链脲佐菌素（65mg/kg）在哥本哈根大鼠中建立糖尿病模型，同时通过向动物皮下接种2×10 Mat-LyLu细胞诱导前列腺癌。在实验期结束时，收集与前列腺功能关系密切的睾丸组织。组织学评估发现，糖尿病和癌症进程后睾丸有中度至重度损伤。在前列腺癌早期观察到组织病理学和生化损伤，在糖尿病动物中这些损伤有所增加。给予二甲双胍可逆转这些损伤，并为睾丸提供实质性保护。特别是，二甲双胍对组织损伤、细胞凋亡、氧化应激和抗氧化能力具有保护作用。这表明，二甲双胍应作为一种针对前列腺癌相关睾丸损伤的靶向保护药物进行进一步研究。
