College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, P. R. China.
Engineering Research Center of Dairy Products Quality and Safety Control Technology, Ministry of Education, Inner Mongolian University, Hohhot, P. R. China.
J Sep Sci. 2022 Feb;45(3):717-727. doi: 10.1002/jssc.202100802. Epub 2021 Dec 16.
In recent years, natural deep eutectic solvents have been favored greatly due to their environment friendly, mild biological toxicity and simple biodegradability. Natural deep eutectic solvents gradually applied for the extracting bioactive compounds from natural products efficiently. In this study, 20 natural deep eutectic solvents were prepared and their physical and chemical properties were tested. The ultrasonic-assisted extraction method was used to extract flavonoids from Trollius ledebouri and high-performance liquid chromatography-ultraviolet was applied to examine two main bioactive flavonoids (orientin and vitexin). Compared with traditional solvents (water and 60% ethanol solution), natural deep eutectic solvents composed of L(-)-proline and levulinic acid (molar ratio 1:2) show a super extraction efficiency. On this basis, the response surface method was used to optimize the extraction temperature, extraction time, water contents, and solid-liquid ratio. As a consequence, the extraction temperature 60℃, extraction time 18 min, water content 14% (v/v), and the solid-liquid ratio 48 mL·g were chosen as the best extraction process. This study shows that natural deep eutectic solvents can effectively extract flavonoids from T. ledebouri, laying a foundation for the further application of natural deep eutectic solvents to extract bioactive compounds from natural products.
近年来，由于其环境友好、生物毒性温和、简单可生物降解等特点，天然深共晶溶剂受到了极大的关注。天然深共晶溶剂逐渐应用于从天然产物中高效提取生物活性化合物。本研究制备了 20 种天然深共晶溶剂，并测试了它们的物理化学性质。采用超声辅助提取法从蓝盆花中提取黄酮类化合物，采用高效液相色谱-紫外法检测两种主要的生物活性黄酮类化合物（木犀草素和荭草苷）。与传统溶剂（水和 60%乙醇溶液）相比，由 L(-)-脯氨酸和乙酰丙酸（摩尔比 1:2）组成的天然深共晶溶剂表现出超高效提取效率。在此基础上，采用响应面法对提取温度、提取时间、含水量和固液比进行优化。结果表明，最佳提取工艺条件为：提取温度 60℃，提取时间 18 min，含水量 14%（v/v），固液比 48 mL·g。本研究表明，天然深共晶溶剂可有效从蓝盆花中提取黄酮类化合物，为进一步将天然深共晶溶剂应用于从天然产物中提取生物活性化合物奠定了基础。
