Shu Penghua, Wang Na, Meng Yu, Fan Shuxian, Liu Simin, Fan Xinfeng, Hu Di, Yin Hao, Wang Huiying, Wang Xin, Wu Mengjiao, Yu Feng, Wei Xialan, Zhang Lin, Huang Jihong
Food and Pharmacy College, Xuchang University, Xuchang 461000 Henan, China; Collaborative Innovation Center of Functional Food by Green Manufacturing, Xuchang 461000 Henan, China; School of Life Sciences, Henan University, Kaifeng 475004 Henan, China.
Food and Pharmacy College, Xuchang University, Xuchang 461000 Henan, China; Collaborative Innovation Center of Functional Food by Green Manufacturing, Xuchang 461000 Henan, China.
Ultrason Sonochem. 2025 Aug 26;121:107535. doi: 10.1016/j.ultsonch.2025.107535.
This study pioneers a green, efficient ultrasound-assisted extraction (UAE) technique using a deep eutectic solvent (DES) to isolate polysaccharides from Cercis chinensis bark, overcoming limitations of conventional methods. Through systematic screening of 15 DES systems, DES-11 (L-proline:ethylene glycol, 1:2 M ratio) was identified as optimal, achieving a polysaccharide yield of 64.7 mg/g-3.7-fold higher than water extraction. Extraction conditions were optimized via response surface methodology (RSM) to 39 % water content, 45 °C, 28 min, solid-liquid ratio 1:40 g/mL, and 240 W ultrasonic power. SEM analysis confirmed that DES-11 disrupts plant cell walls, enhancing mass transfer. Kinetic studies revealed the process follows pseudo-first-order kinetics (R > 0.90) and is primarily controlled by intraparticle diffusion, with an apparent activation energy of 10.28 kJ·mol. The DES-11 extracted polysaccharides exhibited moderate antioxidant activity, with IC values of 0.54 mg/mL (DPPH), 0.24 mg/mL (ABTS) and 0.62 mg/mL (OH), suggesting potential as natural food preservatives. DES recyclability was validated over five cycles, retaining > 78 % efficiency. This work establishes DES-UAE as a sustainable strategy for high-yield polysaccharide extraction, with mechanistic insights into kinetics and diffusion control.
本研究开创了一种绿色、高效的超声辅助提取（UAE）技术，使用深共熔溶剂（DES）从紫荆树皮中分离多糖，克服了传统方法的局限性。通过对15种DES体系的系统筛选，确定DES-11（L-脯氨酸：乙二醇，摩尔比1:2）为最佳体系，多糖产率达到64.7 mg/g，比水提取法高3.7倍。通过响应面法（RSM）将提取条件优化为含水量39%、45℃、28分钟、固液比1:40 g/mL和超声功率240 W。扫描电子显微镜（SEM）分析证实，DES-11破坏了植物细胞壁，增强了传质。动力学研究表明，该过程遵循准一级动力学（R>0.90），主要受颗粒内扩散控制，表观活化能为10.28 kJ·mol。DES-11提取的多糖表现出中等抗氧化活性，DPPH的IC值为0.54 mg/mL、ABTS的IC值为0.24 mg/mL、OH的IC值为0.62 mg/mL，表明其有作为天然食品防腐剂的潜力。DES的可循环性在五个循环中得到验证，效率保持>78%。这项工作确立了DES-UAE作为一种可持续的高产多糖提取策略，并对动力学和扩散控制提供了机理见解。
