约旦患者中细胞色素 7A1 和 ATP 结合盒 G8 基因型与 2 型糖尿病的关联。
The association of cytochrome 7A1 and ATP-binding cassette G8 genotypes with type 2 diabetes among Jordanian patients.
机构信息
Department of Pharmacology, Faculty of Medicine, University of Jordan, Amman, Jordan.
Department of Pharmaceutical Science, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan.
出版信息
Drug Metab Pers Ther. 2021 Nov 29;37(2):149-154. doi: 10.1515/dmpt-2021-0164.
OBJECTIVES
Increased cholesterol levels were found to be associated with diabetes mellitus type II (DM2). The cholesterol is metabolized by cytochrome 7A1 (CYP7A1) and transported in the intestine by ATP-binding cassette G8 (ABCG8). Genetic variants in and genes can affect the cholesterol levels. The aim of this study is to compare the frequency of and and genotypes between healthy and DM2 subjects from Jordanian population.
METHODS
A total of 117 DM2 patients and 100 healthy controls, of Jordanian Arabic origin, were genotyped for and and genetic variants using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism technique.
RESULTS
The study showed that homozygosity of (A-204C) genotype in was significantly higher in DM2 patients (ANOVA, p<0.05) with an odd ratio of 2.66, but (G55C) and (C1199A) genetic polymorphisms in were found in comparable frequencies in both healthy and DM2 subjects.
CONCLUSIONS
The results of this study indicate that genetic polymorphism is associated with DM2. Further clinical studies are required to confirm this finding among DM2 patients of Jordanian origin.
目的
研究发现胆固醇水平升高与 II 型糖尿病（DM2）有关。胆固醇由细胞色素 7A1（CYP7A1）代谢，并由三磷酸腺苷结合盒 G8（ABCG8）在肠道中转运。和基因中的遗传变异可影响胆固醇水平。本研究旨在比较约旦人群中健康个体和 DM2 患者中 和 基因型的频率。
方法
采用聚合酶链反应（PCR）后限制性片段长度多态性技术，对 117 例 DM2 患者和 100 例约旦阿拉伯裔健康对照者的 和 基因变异进行了基因型分析。
结果
研究表明，DM2 患者中 （A-204C）基因型的纯合子明显更高（ANOVA，p<0.05），比值比为 2.66，而 （G55C）和 （C1199A）的遗传多态性在健康和 DM2 患者中频率相似。
结论
本研究结果表明， 基因多态性与 DM2 相关。需要进一步的临床研究来证实约旦 DM2 患者的这一发现。
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